Characteristics of Proinflammatory Cytokines and Chemokines in Airways of Asthmatics: Relationships with Disease Severity and Infiltration of Inflammatory Cells.
Characteristics of Proinflammatory Cytokines and Chemokines in Airways of Asthmatics: Relationships with Disease Severity and Infiltration of Inflammatory Cells.
复制标题
哮喘患者气道促炎细胞因子和趋化因子的特征：与疾病严重程度和炎症细胞浸润的关系
DOI：
10.4103/0366-6999.213428
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发表时间：
2017-09-05
影响因子：
6.1
通讯作者：
Wang C
中科院分区：
文献类型：
--
作者：
Yang T;Li Y;Lyu Z;Huang K;Corrigan CJ;Ying S;Wang W;Wang C
背景：促炎细胞因子和趋化因子的增加可能有助于炎症细胞的浸润和哮喘气道的重塑。尽管这些分子可能与哮喘有关，但缺乏系统证据表明这些事件在疾病中的重要性和重要性。我们的目的是分析一大群哮喘患者（n = 70，包括 37 名轻度哮喘患者和 33 名中度/重度哮喘患者）与对照组（n = 30）相比，这些分子在气道中的浓度以及与疾病严重程度和气道炎症细胞浸润的关系。方法：使用细观尺度发现系统和商业ELISA试剂盒测量促炎细胞因子白细胞介素（IL）-1&bgr;的浓度；肿瘤坏死因子-α（TNF-α）； IL-6;哮喘患者和对照组的支气管肺泡灌洗液中的 IL-17 和 CC 和 CXC 趋化因子 CCL2、CCL4、CCL11、CCL13、CCL17、CCL22 和 CCL26 以及 CXCL8、CXCL9、CXCL10 和 CXCL11。结果：与对照组相比，哮喘患者IL-1、TNF-α、IL-6、CXCL8和CXCL10以及CCL4、CCL11、CCL17和CCL22浓度显着升高（P < 0.05）。 TNF-α的浓度为：和 CXCL8 与疾病严重程度（肺功能 1 秒用力呼气量）呈负相关（TNF-α 与总体：r = -0.359，P = 0.002 与中/重度：r = -0.541，P = 0.001；CXCL8 与总体：r = -0.327，P = 0.006中度/重度：r = -0.625，P = 0.0001，分别）。此外，这两种分子的浓度还与哮喘气道中浸润的嗜酸性粒细胞和中性粒细胞的绝对数量相关。结论：TNF-α浓度增加。 CXCL8和CXCL8与哮喘的发病机制相关。针对这些分子可能为这种疾病提供一种替代疗法。
Background: Increased proinflammatory cytokines and chemokines might contribute to infiltration of inflammatory cells and remodeling in airways of asthma. Although these molecules may be associated with asthma, there is lack of systemic evidence showing which and how important these events are in the disease. We aimed to analyze the concentrations of these molecules in the airways and relationships with disease severity and with airway infiltration of inflammatory cells in a large cohort of asthmatics (n = 70, including 37 mild and 33 moderate/severe asthmatics) compared with controls (n = 30). Methods: Meso scale discovery system and commercial ELISA kits were used to measure the concentrations of proinflammatory cytokines interleukin (IL)-1&bgr;; tumor necrosis factor-alpha (TNF-&agr;); IL-6; and IL-17 and CC and CXC chemokines CCL2, CCL4, CCL11, CCL13, CCL17, CCL22, and CCL26 and CXCL8, CXCL9, CXCL10, and CXCL11 in bronchoalveolar lavage fluid of asthmatics and controls. Results: The concentrations of IL-1, TNF-&agr;, IL-6, CXCL8 and CXCL10, and CCL4, CCL11, CCL17, and CCL22 were significantly elevated in asthmatics compared with controls (P < 0.05). The concentrations of TNF-&agr; and CXCL8, but not others, were negatively correlated with severity of disease (lung function forced expiratory volume in 1 s) (TNF-&agr; vs. total: r = −0.359, P = 0.002 vs. moderate/severe: r = −0.541, P = 0.001; CXCL8 vs. total: r = −0.327, P = 0.006 vs. moderate/severe: r = −0.625, P = 0.0001, respectively). In addition, concentrations of these two molecules were also correlated with the absolute numbers of infiltrating eosinophils and neutrophils in asthmatic airways. Conclusions: Increased concentrations of TNF-&agr; and CXCL8 are associated with pathogenesis of asthma. Targeting these molecules might provide an alternative therapeutic for this disease.