FAC1 expression and localization in motor neurons of developing, adult, and amyotrophic lateral sclerosis spinal cord.
FAC1 expression and localization in motor neurons of developing, adult, and amyotrophic lateral sclerosis spinal cord.
复制标题
FAC1 在发育中、成人和肌萎缩侧索硬化症脊髓运动神经元中的表达和定位。
DOI：
10.1006/exnr.1997.6508
复制
发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Bowser,R
中科院分区：
文献类型：
--
作者：
Mu,X;Springer,JE;Bowser,R
在这项研究中，我们报道了FAC1蛋白在发育中、正常成人和肌萎缩侧索硬化症（ALS）腰椎中的定位和表达。在发育中的腰椎所有区域（灰质和白质）的细胞中检测到高水平的FAC1蛋白。在脊髓发育早期，FAC1蛋白主要定位于运动神经元的细胞核和细胞体中。相比之下，在成人腰椎脊髓中观察到低水平的FAC1蛋白，仅局限于IX层大α运动神经元的细胞体中。有趣的是，与对照组相比，存活的ALS脊髓运动神经元中的FAC1蛋白表达升高，并且位于运动神经元的细胞核和整个细胞质中。在ALS脊髓白质细胞和纤维中也观察到FAC1蛋白。为了支持免疫细胞化学结果，原位杂交研究表明，FAC1 mRNA在ALS脊髓运动神经元中也升高。这些数据通过免疫细胞化学技术描述了脊髓中FAC1蛋白的发育调控，并提供了该蛋白在ALS运动神经元中重新表达的证据。
In this study we report the localization and expression of FAC1 protein in developing, normal adult and amyotrophic lateral sclerosis (ALS) lumbar spinal cord. High levels of FAC1 protein were detected in cells throughout all areas (gray and white matter) of the developing lumbar spinal cord. FAC1 protein was localized predominately in nuclei and the cell body of motor neurons during early stages of spinal cord development. In contrast, low levels of FAC1 protein were observed in the adult lumbar spinal cord, localized only in the cell body of large alpha motor neurons found in lamina IX. Interestingly, FAC1 protein expression was elevated in surviving motor neurons of ALS spinal cord compared to the controls and was located both in the nucleus and throughout the cytoplasm of motor neurons. FAC1 protein was also observed in white matter cells and fibers in ALS spinal cord. In support of the immunocytochemical results,in situhybridization studies demonstrated that FAC1 mRNA is also elevated in ALS spinal cord motor neurons. These data describe the developmental regulation of FAC1 protein in the spinal cord by immunocytochemical techniques and provide evidence that this protein is reexpressed in ALS motor neurons.