Anti-bilirubin monoclonal antibody. I. Preparation and properties of monoclonal antibodies to covalently coupled bilirubin-albumin.
Anti-bilirubin monoclonal antibody. I. Preparation and properties of monoclonal antibodies to covalently coupled bilirubin-albumin.
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抗胆红素单克隆抗体。
DOI：
10.1016/0304-4165(88)90017-7
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
H. Nakajima
中科院分区：
文献类型：
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作者：
S. Shimizu;Y. Izumi;M. Yamazaki;K. Shimizu;T. Yamaguchi;H. Nakajima
我们制备了16个抗胆红素单克隆抗体，并描述了它们对胆红素及其相关化合物的独特反应性。采用改进的混合酸酐法，将胆红素共价偶联到牛血清白蛋白上。我们将用这种血红蛋白-白蛋白复合物免疫的小鼠脾细胞与小鼠骨髓瘤P3-X63-Ag 8-U1细胞进行体细胞融合。筛选试验后，鉴定出16个克隆，它们产生的抗体不是针对白蛋白的，而是针对半抗原胆红素的。在抑制分析中，培养上清液中的抗体与胆红素葡萄糖醛酸苷发生不同程度的交叉反应，但很少与胆红素的结构相关的胆绿素、氯化血红素和偶氮二吡咯反应。当白蛋白存在于溶液中时，大大降低了几种单克隆抗体对未结合胆红素的反应性，并且通过加入水杨酸盐（其使胆红素和白蛋白之间的结合解离）可部分逆转这种效应。
We have prepared 16 anti-bilirubin monoclonal antibodies and described their unique reactivity to bilirubin and related compounds. Using the modified mixed anhydride method, bilirubin was covalently coupled to bovine serum albumin. We performed somatic cell fusion between murine spleen cells immunized with this bilirubin-albumin complex and murine myeloma P3-X63-Ag8-U1 cells. After screening assays, 16 clones were identified which were producing antibodies not to albumin but to haptenic bilirubin. In inhibition analysis, the antibodies in the culture supernatants cross-reacted with bilirubin glucuronides to varying degrees, but rarely reacted with structurally related biliverdin, hemin, and azodipyrroles of bilirubin. Albumin, when present in the solution, much reduced the reactivity of several monoclonal antibodies to unconjugated bilirubin, and this effect was partly reversed by addition of salicylate which dissociates the binding between bilirubin and albumin.