Neurocan contributes to the molecular heterogeneity of the perinodal ECM
Neurocan contributes to the molecular heterogeneity of the perinodal ECM
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DOI：
10.1679/aohc.73.95
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发表时间：
2010-01-01
影响因子：
--
通讯作者：
Oohashi, Toshitaka
中科院分区：
文献类型：
--
作者：
Bekku, Yoko;Oohashi, Toshitaka
Neurocan是凝集素家族的中枢神经组织特异性硫酸软骨素蛋白聚糖。它主要在该组织的建模和重塑阶段表达，被认为通过与各种细胞外基质和细胞组分结合发挥重要作用。在成年人中，神经聚糖表达与神经元周网结构相关。本研究显示了神经聚糖在小鼠中枢神经组织中郎维结的免疫定位。在视神经中检测到的主要形式是N-末端片段neurocan（Ncan 130）。在脑组织和神经束的白色物质中，神经聚糖的表达与其他结周细胞外基质分子多功能蛋白聚糖V2和短蛋白聚糖的表达相比显示出差异表达谱。双免疫标记的neurocan和结标记，麸皮，或paranodal标记，半胱氨酸蛋白酶，表明，neurocan是本地化的节点的郎维尔。在许多-而不是所有-淋巴结区域发现神经聚糖表达，并且神经聚糖阳性淋巴结数量超过短蛋白聚糖阳性淋巴结。在Bral 1缺陷小鼠中，神经聚糖的淋巴结定位减少。总之，这些发现表明，神经蛋白聚糖有助于结周基质的分子异质性，其结的表达依赖于Bral 1。
Neurocan is a central nervous tissue-specific chondroitin sulfate proteoglycan of the lectican family. Mainly expressed during modeling and remodeling stages of this tissue, it is thought to play an important role via binding to various extracellular matrix and cellular components. In adults, neurocan expression is associated with the perineuronal net structures. This study shows the neurocan immunolocalization at the node of Ranvier in mouse central nervous tissues. The N-terminal fragment of neurocan (Ncan130) was the predominant form detected in the optic nerve. The expression of neurocan in the white matter of brain tissue and nerve tracts revealed differential expression profiles compared with those of versican V2 and brevican, other perinodal extracellular matrix molecules. Double immunolabeling for neurocan and a nodal marker, Bran, or a paranodal marker, caspr, demonstrated that neurocan was localized at the node of Ranvier. Neurocan expression was found at many-not all-nodal regions, and neurocan-positive nodes outnumbered brevican-positive nodes. The nodal localization of neurocan was diminished in Bral1-deficient mice. Taken together, these findings indicate that neurocan contributes to the molecular heterogeneity of the perinodal matrix, and its nodal expression is dependent on Bral1.