Interactions of Apolipoproteins with Lipid-Based Nanoparticles
Interactions of Apolipoproteins with Lipid-Based Nanoparticles
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DOI：
10.1021/acsnano.2c10790
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发表时间：
2023-01-09
期刊：
影响因子：
17.1
通讯作者：
Isaac, Sami
中科院分区：
文献类型：
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作者：
Dalhaimer, Paul;Florey, Brice;Isaac, Sami
血清蛋白质结合在注射到哺乳动物血管中的纳米颗粒(NPs)周围，并形成一个动态的蛋白质日冕。一些基础研究表明，载脂蛋白是NP日冕的重要组成部分。由于载脂蛋白控制脂蛋白的分布，它们也可能控制NPs的分布。事实上，载脂蛋白与NPs的亲和力最近被利用来将包裹在NPs中的CRISPR试剂输送到表达特定脂蛋白受体的细胞。在这种情况下，载脂蛋白与NP结合，由此产生的载脂蛋白-NP复合体与表达(Apo)脂蛋白受体的细胞结合。但是，如果NP不表达(Apo)脂蛋白受体，它就会从靶细胞转移。这可能会阻碍NP对疾病的治疗。因此，我们必须了解载脂蛋白-NP亲和力的动力学，以及载脂蛋白-NP相互作用如何影响NP的生物分布。在这个视角下，我们讨论了载脂蛋白NP相互作用的不断演变的话题，这对所有基于NP的疾病治疗都非常有意义。载脂蛋白-NP复合体的许多性质尚未确定，并将在动物模型和临床上对许多基于NP的治疗效果产生重大影响。
Serum proteins bind and form a dynamic protein corona around nanoparticles (NPs) that have been injected into the mammalian vasculature. Several fundamental studies have shown that apolipoproteins are prominent components of the NP corona. Since apolipoproteins control the distribution of lipoproteins, they may also control the distribution of NPs. Indeed, apolipoprotein affinity for NPs has been recently taken advantage of to deliver CRISPR reagents encapsulated in NPs to cells that express particular lipoprotein receptors. In this scenario, an apolipoprotein binds an NP and the resulting apolipoprotein-NP complex binds a cell that expresses the (apo)lipoprotein receptor. But the NP will be diverted from the target cell if it does not express the (apo)lipoprotein receptor. This may hamper NP treatment of diseases. Therefore, we must understand the kinetics of apolipoprotein-NP affinity and how apolipoprotein-NP interactions affect NP biodistribution. In this Perspective, we discuss the evolving topic of apolipoproteinNP interactions, which is of great interest for all NP-based disease treatments. Many properties of apolipoprotein-NP complexes are yet to be determined and will have a significant impact on NP efficacy for many NP-based treatments in animal models and in the clinic.