Altered expression of Smad family members in injured motor neurons of rat
Altered expression of Smad family members in injured motor neurons of rat
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DOI：
10.1016/j.brainres.2006.11.019
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发表时间：
2007-02
期刊：
影响因子：
2.9
通讯作者：
N. Okuyama;S. Kiryu-Seo;H. Kiyama
中科院分区：
文献类型：
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作者：
N. Okuyama;S. Kiryu-Seo;H. Kiyama
我们检测了Smad家族成员在舌下神经损伤后表达的变化，Smad家族成员转导TGF-β超家族配体的信号。RT-PCR和原位杂交显示Smad1、2、3和4 mRNA在损伤侧显著上调，而Smad8 mRNA下调。免疫组织化学和Western blotting分析证实Smad1、2和4在损伤神经元中发生改变。这些结果表明Smad信号可能对神经再生很重要。
We examined changes in the expression of Smad family members, which transduce signals from TGF-β superfamily ligands, following hypoglossal nerve injury. RT–PCR and in situ hybridization revealed that Smad1, 2, 3 and 4 mRNAs were significantly up-regulated in injured side, whereas Smad8 mRNA was down-regulated. Immunohistochemistry and Western blotting analysis confirmed the alterations of Smad1, 2 and 4 in injured neurons. These results suggest that the Smad signaling may be important for nerve regeneration.