Expression of genes involved in proanthocyanidin biosynthesis during fruit development in a Chinese pollination-constant, nonastringent (PCNA) persimmon, 'Luo Tian Tian Shi'.
Expression of genes involved in proanthocyanidin biosynthesis during fruit development in a Chinese pollination-constant, nonastringent (PCNA) persimmon, 'Luo Tian Tian Shi'.
复制标题
DOI：
10.21273/jashs.130.6.830
复制
发表时间：
2005-11-01
影响因子：
1.9
通讯作者：
Yamada, M
中科院分区：
文献类型：
--
作者：
Ikegami, A;Yonemori, K;Yamada, M
缩合单宁（CT）相关基因的表达模式。研究了中国常授粉甜柿（Diospyros kaki Thunb.）“罗天天师”。‘罗田甜食’的苯丙氨酸解氨酶（PAL）和二氢黄酮醇还原酶（DFR）在果实生长过程中一直处于高水平。查尔酮合成酶（CHS）和黄酮醇3-羟化酶（F3 H）在果实生长过程中也继续转录，但其水平下降早于PAL和DFR。与此相反，这些基因的表达水平下降到不可检测的水平在果实发育的早期阶段，在日本的PCNA柿。此外，原花色素合成的关键酶花青素还原酶（ANR）在'罗天甜柿'果实生长过程中高水平转录，而在日本柿PCNA中不转录。相比之下，D.通过抑制性消减杂交（SSH）分析，获得了柿丝氨酸羧肽酶样蛋白1（DkSCPL 1）基因，该基因在“罗田甜柿”中比其它类黄酮合成基因早衰退，与单宁细胞发育的终止相一致。在‘罗田田氏’和日本PCNA‘太树’杂交的F_1代中，分离的PCNA和涩味后代中获得了相似的表达模式。这些结果表明，中国和日本的PCNA在CT生物合成相关基因的表达上存在差异。总之，我们澄清，参与类黄酮生物合成的基因（PAL ANR，但不SCPL）的表达是连续的中国PCNA品种，尽管单宁细胞发育的终止。
Expression patterns of the genes involved in condensed tannin (CT). biosynthesis during fruit development was investigated in a Chinese pollination-constant, nonastringent (PCNA) persimmon (Diospyros kaki Thunb.) 'Luo Tian Tian Shi'. The transcript levels of phenylalanine ammonia-lyase (PAL) and dihydroflavonol reductase (DFR) in 'Luo Tian Tian Shi' were detected at high levels throughout the fruit growth. Chalcone synthase (CHS) and flavonol 3-hydroxylase (F3H) also continued to be transcribed during fruit growth, although their levels decreased earlier than PAL and DFR. In contrast, expression levels of these genes declined into undetectable levels at an early stage of fruit development in Japanese PCNA persimmon. In addition, anthocyanidin reductase (ANR), which encodes a key enzyme of the proanthocyanidin biosynthesis, was transcribed at high levels in 'Luo Tian Tian Shi'during fruit growth, but not in Japanese PCNA persimmon. By contrast, the expression of D. kaki serine carboxypeptidase-like protein 1 (DkSCPL1) that was obtained from suppression subtractive hybridization (SSH) analysis between artificially astringency-removed fruit and astringent fruit in a different experiment, declined earlier than the other flavonoid biosynthesis genes in 'Luo Tian Tian Shi', coincident with the termination of the tannin cell development. In the F, progeny of the cross between 'Luo Tian Tian Shi'and Japanese PCNA 'Taishu', similar expression patterns were obtained among segregated PCNA and astringent offspring. These results indicate that Chinese PCNA is different from Japanese PCNA in expression of the genes involved in CT biosynthesis. In conclusion, we clarified that expression of the genes (PAL to ANR, but not SCPL) involved in flavonoid biosynthesis was continuous in the Chinese PCNA cultivar, despite the termination of tannin cell development.