L-Cysteine: A biocompatible, breathable and beneficial coating for graphene oxide
L-Cysteine: A biocompatible, breathable and beneficial coating for graphene oxide
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L-半胱氨酸：一种生物相容性、透气性和有益的氧化石墨烯涂层
DOI：
10.1016/j.biomaterials.2015.02.046
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发表时间：
2015-06-01
期刊：
影响因子：
14
通讯作者：
Hu, Xiangang
中科院分区：
文献类型：
--
作者：
Mu, Li;Gao, Yue;Hu, Xiangang
氧化石墨烯（GO）已应用于各个领域，其生态和健康风险备受关注。将小而便宜的生物分子L-半胱氨酸共价固定到GO上以形成厚度为1.4nm的硫代官能化纳米片的L-半胱氨酸-GO（CysGO）。观察到CysGO的d间距和结晶度均低于GO的d间距和结晶度，而D峰和G峰保持与GO的D峰和G峰相似。CysGO在斑马鱼胚胎中表现出显著的体内摄取：未观察到组织缺陷、畸形、死亡或显著的孵化延迟。CysGO未观察到显著的DNA损伤、Na+/K+ ATP酶活性降低和线粒体膜电位降低。作为与纳米毒理学相关的非特异性活性，CysGO的未配对电子自旋强度比GO低大约两个数量级。氧微传感器分析表明，分别由GO和CysGO修饰物的存在导致的低氧和常氧环境导致生物相容性的差异。CysGO还可以保护胚胎免受砷中毒。因此，CysGO具有GO的有利性质，表现出优异的生物相容性，充当透气涂层和解毒剂，并且适用于各种应用。(C)2015爱思唯尔有限公司版权所有。
Graphene oxide (GO) has been employed in various fields, and its ecological and health risks have attracted much attention. A small and inexpensive biomolecule, L-cysteine, was covalently immobilized onto GO to form L-cysteine-GO (CysGO) as a thio-functionalized nanosheet of 1.4 nm in thickness. Both the d-spacing and crystallinity of CysGO were observed to be lower than those of GO, whereas the D and G peaks remained similar to those of GO. CysGO exhibited remarkable uptake in vivo: no tissue defects, malformation, death or significant hatching delay were observed in zebrafish embryos. Significant DNA damage, decreased Na+/K+ ATPase activity and decreased mitochondria] membrane potential were not observed for CysGO. As a nonspecific activity linked to nanotoxicology, the unpaired electron spinning intensity of CysGO was approximately two orders of magnitude lower than that of GO. Oxygen microsensor analysis showed that the hypoxic and normoxic environments resulting from the presence of GO and CysGO envelopment, respectively, contributed to the difference in biocompatibility. CysGO also protected embryos from arsenic poisoning. Thus, CysGO has the advantageous properties of GO, exhibits excellent biocompatibility, acts as a breathable coating and antidote, and is suitable for various applications. (C) 2015 Elsevier Ltd. All rights reserved.