Label-Free Electrochemical Detection of MicroRNAs via Intercalation of Hemin into the DNA/RNA Hybridization
Label-Free Electrochemical Detection of MicroRNAs via Intercalation of Hemin into the DNA/RNA Hybridization
复制标题
通过将氯化血红素插入 DNA/RNA 杂交中对 MicroRNA 进行无标记电化学检测
DOI：
10.20964/2017.04.33
复制
发表时间：
2017-04
影响因子：
1.5
通讯作者：
Chen Zhuo
中科院分区：
文献类型：
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作者：
Yi Xinyao;Lu Zhixuan;Kong Yu;Chen Zhuo
MicroRNAs (miRNAs) have been emerged as new biomarkers for the detection of a wide variety of cancers. On the basis of the employment of hemin as electron transfer mediator and signal unit, a simple electrochemical assay for sensitive and selective determination of miRNA has been developed. In the presence of miRNA, hemin can intercalate into the hybridization between the preimmobilized DNA probe and miRNA, thus lead to a reasonable electrochemical signal. In the absence of miRNA, hemin cannot intercalate into the single strand DNA probe which lead to undetectable signal. The current is proportional to the concentration of miRNA in a range between 0.1 μM and 1μM. The proposed assay is label-free, simple and highly selective toward the target miRNA and could be an alternative for detection of miRNA in clinic samples.
DOI：
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发表时间：
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期刊：
--
影响因子：
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作者：
MD YOSHIO KINAMI;I. Miyazaki;Mdi
通讯作者：
MD YOSHIO KINAMI;I. Miyazaki;Mdi