A polymorphism in the coding region of interleukin-13 gene is associated with atopy but not asthma in Chinese children
A polymorphism in the coding region of interleukin-13 gene is associated with atopy but not asthma in Chinese children
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DOI：
10.1046/j.1365-2222.2001.01212.x
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发表时间：
2001-10-01
影响因子：
6.1
通讯作者：
Lam, CWK
中科院分区：
文献类型：
--
作者：
Leung, TF;Tang, NLS;Lam, CWK
背景白细胞介素13（IL-13）是由2型辅助性T淋巴细胞分泌的一种重要细胞因子。对于调节人B细胞的IgE合成是必需的。以往的研究表明，IL-13基因多态性与血清总IgE或过敏性哮喘相关。目的探讨IL-13基因编码区的一个多态性是否与香港地区哮喘儿童的哮喘和特应性有关。方法采用限制性片段长度多态性技术检测中国哮喘儿童和对照组的R130 Q基因型。采用微粒子免疫法测定血清总IgE，荧光酶免疫法测定常见吸入性变应原特异性IgE。肺功能的研究进行了肺功能测定。结果157例患者和54名对照儿童被招募。血清总IgE浓度分别为994 kIU/L和473 kIU/L（P < 0.0001）。在141名患者和32名对照儿童中发现了特应性（定义为1例阳性RAST）。IL-13基因R130 Q多态性的GlnGln形式与血清总IgE（P = 0.005）以及对Der p 1（P = 0.021）、混合蟑螂（P = 0.03）和狗（P = 0.003）的特异性IgE相关，但与医生诊断的哮喘无关（P = 0.621）。结论IL-13基因R130 Q多态性与中国儿童血清总IgE和过敏原特异性IgE升高有关，但与哮喘无关。
Background Interleukin (IL)-13 is an important cytokine secreted from type 2 helper T lymphocytes. It is essential for modulating IgE synthesis by human B cells. Previous studies showed that polymorphisms in the IL-13 gene were associated with serum total IgE or allergic asthma. The relationship of this marker with sensitization to individual aeroallergens has not been evaluated.Objective We tested whether a polymorphism in the coding region of the IL-13 gene is associated with asthma and atopy in asthmatic children in Hong Kong.Methods We used restriction fragment length polymorphism to detect R130Q genotype in Chinese children with asthma and control subjects. Serum total IgE was measured by microparticle immunoassay and specific IgE to common aeroallergens was measured using fluorescent enzyme immunoassay. Pulmonary function studies were performed using spirometry.Results One hundred and fifty-seven patients and 54 control children were recruited. Their mean serum total IgE concentrations were 994 kIU/L and 473 kIU/L, respectively (P < 0.0001). Atopy as defined by 1 positive RAST was found in 141 patients and 32 control children. The GlnGln form of the R130Q polymorphism in the IL-13 gene was associated with serum total IgE (P = 0.005) as well as specific IgE to Der p 1 (P = 0.021), mixed cockroaches (P = 0.03) and dog (P = 0.003) but not with physician-diagnosed asthma (P = 0.621). In addition, the R130Q polymorphism did not correlate with subjective or objective indicators of asthma severity in our patients.Conclusion Our results suggest that the R130Q polymorphism of the IL-13 gene is associated with elevated serum total and allergen-specific IgE but not asthma in Chinese children.