Black soybean seed coat polyphenols promote nitric oxide production in the aorta through glucagon-like peptide-1 secretion from the intestinal cells
Black soybean seed coat polyphenols promote nitric oxide production in the aorta through glucagon-like peptide-1 secretion from the intestinal cells
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DOI：
10.1039/c9fo02050k
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发表时间：
2019-12-01
期刊：
影响因子：
6.1
通讯作者：
Yamashita, Yoko
中科院分区：
文献类型：
--
作者：
Domae, Chiaki;Nanba, Fumio;Yamashita, Yoko
黑豆种皮多酚具有多种生物调节功能。然而，黑豆种皮多酚对血管功能的影响尚不清楚。由衰老和血管僵硬引起的血管功能障碍与心血管疾病（CVD）的风险相关，一氧化氮（NO）水平的降低可引发CVD的发作。在本研究中，我们研究了富含多酚的黑豆种皮提取物（BE）对血管功能的影响及其相关机制。通过eNOS磷酸化测定，Wistar大鼠经口给予BE 50 mg/kg体重可增加NO水平。BE的给药也增加了GLP-1和cAMP水平。此外，在GLP-1受体拮抗剂的存在下，BE的作用被抑制。这表明GLP-1强烈参与体内NO产生的潜在机制。总之，BE通过促进NO的产生而有助于改善血管功能。关于推定的潜在机制，由BE中的多酚从肠细胞分泌的GLP-1激活血管内皮细胞中的eNOS。
Black soybean seed coat polyphenols were reported to possess various bioregulatory functions. However, the effects of black soybean seed coat polyphenols on vascular functions are unknown. Vascular dysfunction caused by aging and vascular stiffness is associated with a risk of cardiovascular disease (CVD), and a reduction in nitric oxide (NO) levels can trigger the onset of CVD. In the present study, we investigated the effect of polyphenol-rich black soybean seed coat extract (BE) on vascular functions and the underlying mechanisms involved. The oral administration of BE at 50 mg per kg body weight to Wistar rats increased NO levels as determined by eNOS phosphorylation. The administration of BE also increased GLP-1 and cAMP levels. Furthermore, the effects of BE were inhibited in the presence of a GLP-1 receptor antagonist. This suggests that GLP-1 is strongly involved in the underlying mechanism of NO production in vivo. In conclusion, BE contributes to the improvement of vascular functions by promoting NO production. Regarding the putative underlying mechanism, GLP-1 secreted from intestinal cells by the polyphenols in BE activates eNOS in vascular endothelial cells.