Genome-wide identification, characterisation, and evolution of ABF/AREB subfamily in nine Rosaceae species and expression analysis in mei (Prunus mume).
Genome-wide identification, characterisation, and evolution of ABF/AREB subfamily in nine Rosaceae species and expression analysis in mei (Prunus mume).
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作者：
Yong X;Zheng T;Zhuo X;Ahmad S;Li L;Li P;Yu J;Wang J;Cheng T;Zhang Q
蔷薇科是一个重要的科，包含一些高度进化的果实和观赏植物。非生物胁迫反应在植物的季节性生长发育中起着关键作用。然而，在蔷薇科植物中，胁迫反应的分子基础在很大程度上仍不清楚。脱落酸(ABA)是一种参与非生物应激反应途径的应激激素。Abre结合因子/ABA反应元件结合蛋白(ABF/AREB)是碱性结构域/亮氨酸拉链(BZIP)转录因子家族的一个亚家族。它在ABA介导的信号通路中发挥着重要作用。在这里，我们分析了9种蔷薇科植物的ABF/AREB亚家族基因。共鉴定到ABF/AREB基因，其中玫瑰亚科、杏仁亚科和马蹄亚科传统亚科分别有18、28和18个基因。通过系统发育分析、基因结构和保守基序组成、Ka/Ks值和种间共线性分析，研究了ABF/AREB亚家族基因的进化关系。这些基因集被聚为四个组。在李ABF/AREB(PmABF)启动子中，预测了几个与光、激素和非生物胁迫反应相关的顺式元件。除PmABF5外，PmABF在五种不同的组织中均有表达，PmABF5仅在芽中表达。在休眠期，PmABF1、PmAB5和PmAB7表达差异。PmABF3、4、6的表达与ABA浓度呈正相关。除PmABF5外，所有PmABF对ABA均敏感。在PmABF1、3、6、7启动子中含有多种ABRE元素。根据我们的研究结果，我们推测PmABF可能在梅花花芽休眠中起作用。
Rosaceae is an important family containing some of the highly evolved fruit and ornamental plants. Abiotic stress responses play key roles in the seasonal growth and development of plants. However, the molecular basis of stress responses remains largely unknown in Rosaceae. Abscisic acid (ABA) is a stress hormone involving abiotic stress response pathways. The ABRE-binding factor/ABA-responsive element-binding protein (ABF/AREB) is a subfamily of the basic domain/leucine zipper (bZIP) transcription factor family. It plays an important role in the ABA-mediated signaling pathway. Here, we analyzed the ABF/AREB subfamily genes in nine Rosaceae species. A total of 64 ABF/AREB genes were identified, including 18, 28, and 18 genes in the Rosoideae, Amygdaloideae, and Maloideae traditional subfamilies, respectively. The evolutionary relationship of the ABF/AREB subfamily genes was studied through the phylogenetic analysis, the gene structure and conserved motif composition, Ka/Ks values, and interspecies colinearity. These gene sets were clustered into four groups. In the Prunus ABF/AREB (PmABF) promoters, several cis-elements related to light, hormone, and abiotic stress response were predicted. PmABFs expressed in five different tissues, except PmABF5, which expressed only in buds. In the dormancy stages, PmABF1, 2, 5 and 7 showed differential expression. The expression of PmABF3, 4 and 6 was positively correlated with the ABA concentration. Except for PmABF5, all the PmABFs were sensitive to ABA. Several ABRE elements were contained in the promoters of PmABF1, 3, 6, 7. Based on the findings of our study, we speculate that PmABFs may play a role in flower bud dormancy in P. mume.