Destructive extraction of phospholipids from Escherichia coli membranes by graphene nanosheets
Destructive extraction of phospholipids from Escherichia coli membranes by graphene nanosheets
复制标题
石墨烯纳米片从大肠杆菌膜中破坏性提取磷脂
DOI：
10.1038/nnano.2013.125
复制
发表时间：
2013-08-01
影响因子：
38.3
通讯作者：
Zhou, Ruhong
中科院分区：
文献类型：
--
作者：
Tu, Yusong;Lv, Min;Zhou, Ruhong
了解纳米材料如何与细胞膜相互作用与它们如何引起细胞毒性有关，因此对于设计更安全的生物医学应用至关重要。最近，石墨烯（一种二维纳米材料）被证明对大肠杆菌具有抗菌活性，但其潜在的分子机制仍然未知。在这里，我们从实验和理论上表明，原始石墨烯和氧化石墨烯纳米片可以诱导大肠杆菌内外细胞膜的降解，并降低其生存能力。透射电子显微镜显示了三个粗略的阶段，分子动力学模拟揭示了这个过程的原子细节。由于石墨烯与脂质分子之间的强分散相互作用，石墨烯纳米片可以渗透到细胞膜中并从细胞膜中提取大量磷脂。这种破坏性的提取为石墨烯的细胞毒性和抗菌活性的分子基础提供了一种新的机制。
Understanding how nanomaterials interact with cell membranes is related to how they cause cytotoxicity and is therefore critical for designing safer biomedical applications. Recently, graphene (a two-dimensional nanomaterial) was shown to have antibacterial activity on Escherichia coli, but its underlying molecular mechanisms remain unknown. Here we show experimentally and theoretically that pristine graphene and graphene oxide nanosheets can induce the degradation of the inner and outer cell membranes of Escherichia coli, and reduce their viability. Transmission electron microscopy shows three rough stages, and molecular dynamics simulations reveal the atomic details of the process. Graphene nanosheets can penetrate into and extract large amounts of phospholipids from the cell membranes because of the strong dispersion interactions between graphene and lipid molecules. This destructive extraction offers a novel mechanism for the molecular basis of graphene's cytotoxicity and antibacterial activity.