PEPTIDE VACCINES DERIVED FROM A MALARIAL SURFACE-ANTIGEN - EFFECTS OF DOSE AND ADJUVANTS ON IMMUNOGENICITY
PEPTIDE VACCINES DERIVED FROM A MALARIAL SURFACE-ANTIGEN - EFFECTS OF DOSE AND ADJUVANTS ON IMMUNOGENICITY
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DOI：
10.1016/0165-2478(90)90008-e
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发表时间：
1990-07-01
影响因子：
4.4
通讯作者：
SAUL, A
中科院分区：
文献类型：
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作者：
JONES, GL;SPENCER, L;SAUL, A
肽P2122（CKNNNSTNSGI）和P513（CSQRSTNSAST）含有抑制性单克隆抗体识别的疟疾表面抗原（MSA 2）表位，将其与白喉类毒素（DT）蛋白偶联，并与各种凝胶和油包水乳液佐剂一起配制在小鼠和兔的疫苗试验中。P2122-DT构建体在产生与免疫肽和天然抗原两者反应的抗体方面是有效的。通过小鼠中抗肽或抗蛋白应答的滴度测量的有效邻接性按以下顺序变化：弗氏佐剂，Montanide伊萨708，721，70 > >明矾，角鲨烯Arlacel > SAF-1。在家兔中观察到相似的佐剂效力顺序：弗氏>明矾>角鲨烯Arlacel > SAF-1。
Peptides P2122 (CKNNNSTNSGI) and P513 (CSQRSTNSAST) containing an epitope of a malarial surface antigen (MSA2) recognised by inhibitory monoclonal antibodies were conjugated to diphtheria toxoid (DT) protein and formulated with various gel-based and water in oil emulsion adjuvants in vaccine trials in mice and rabbits. The P2122-DT construct was effective in raising antibodies reactive with both the immunising peptide and the native antigen. Effective adjunvanticity as measured by the titre of the anti-peptide or anti-protein response in mice varied in the order: Algammulin, Montanide ISA 50 .gtoreq. Freund''s adjuvant, Montanide ISA 708, 721, 70 > > alum, Squalene Arlacel > SAF-1. A similar order of adjuvant efficacy: Freund''s > alum > Squalene Arlacel > SAF-1, was observed in rabbits.