3D functional ultrasound imaging of pigeons
3D functional ultrasound imaging of pigeons
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DOI：
10.1016/j.neuroimage.2018.08.014
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发表时间：
2018-12-01
期刊：
影响因子：
5.7
通讯作者：
Maret, Georg
中科院分区：
文献类型：
--
作者：
Rau, Richard;Kruizinga, Pieter;Maret, Georg
超声多普勒成像的最新进展促进了功能超声（fUS）技术的发展，该技术能够实现神经血管耦合引起的大脑活动的可视化。到目前为止，这项技术已应用于啮齿类动物以及清醒开颅手术期间的人类受试者和人类新生儿。在这里，我们展示了首次对清醒鸽子进行听觉和视觉刺激的成功 fUS 研究。为了对鸽子进行成功的 fUS，我们通过实时可视化和连续记录将 fUS 的时间分辨率提高到每秒 20,000 帧。我们表明，时间分辨率的提高显着提高了检测脑血流量和体积小波动的灵敏度，这可能反映了局部神经活动的增加。通过这种增加的灵敏度，我们能够捕捉由复杂的刺激模式（例如移动光源）引起的复杂的 3D 神经活动模式。通过进一步突破 fUS 的极限，我们重申了该技术作为功能性脑成像新标准的巨大潜力，能够以优异的时空分辨率和宽视野来揭示未知的、刺激相关的血流动力学。
Recent advances in ultrasound Doppler imaging have facilitated the technique of functional ultrasound (fUS) which enables visualization of brain-activity due to neurovascular coupling. As of yet, this technique has been applied to rodents as well as to human subjects during awake craniotomy surgery and human newborns. Here we demonstrate the first successful fUS studies on awake pigeons subjected to auditory and visual stimulation. To allow successful fUS on pigeons we improved the temporal resolution of fUS up to 20,000 frames per second with real-time visualization and continuous recording. We show that this gain in temporal resolution significantly increases the sensitivity for detecting small fluctuations in cerebral blood flow and volume which may reflect increased local neural activity. Through this increased sensitivity we were able to capture the elaborate 3D neural activity pattern evoked by a complex stimulation pattern, such as a moving light source. By pushing the limits of fUS further, we have reaffirmed the enormous potential of this technique as a new standard in functional brain imaging with the capacity to unravel unknown, stimulus related hemodynamics with excellent spatiotemporal resolution with a wide field of view.