Colorimetric detection of protein microarrays based on nanogold probe coupled with silver enhancement
Colorimetric detection of protein microarrays based on nanogold probe coupled with silver enhancement
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DOI：
10.1016/j.jim.2003.11.008
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发表时间：
2004-02-15
影响因子：
2.2
通讯作者：
Ruan, KC
中科院分区：
文献类型：
--
作者：
Liang, RQ;Tan, CY;Ruan, KC
本工作提出了一种利用比色纳米金探针结合银增敏(金银检测)分析蛋白质微阵列的方法。在该方法中，通过金标记抗体或链霉亲和素的特异性结合，将金纳米粒子引入到微阵列中，然后与银增强结合，产生微阵列斑点的黑色图像，这一点可以很容易地被商用CCD摄像机检测到。该方法具有较高的检测灵敏度(1pg/玻片或2.75 ng/mlIgG溶液)，且信号强度与样品浓度的对数呈良好的线性关系。用该方法对本实验室研制的TORCH抗原芯片进行了检测，结果与荧光法一致。这些结果表明，金银比色检测方法在蛋白质组学研究和临床诊断中具有潜在的应用前景。(C)2004爱思唯尔B.V.保留所有权利。
This work presents a method for analyzing protein microarrays using a colorimetric nanogold probe coupled with silver enhancement (gold-silver detection), In this method, the gold nanoparticles were introduced to the microarray by the specific binding of the gold-conjugated antibodies or streptavidins and then coupled with silver enhancement to produce black image of microarray spots, which can be easily detected with a commercial CCD camera. The method showed high detection sensitivity (1 pg of IgG immobilized on slides or 2.75 ng/ml IgG in solution) and a good linear correlation between the signal intensity and the loaarithm of the sample concentration. The examination of this method in analyzing a demonstrational ToRCH antigen microarray developed in our lab showed an identical result as in the fluorescent method. These results suggest the colorimetric gold-silver detection method has potential applications in proteomics research and clinical diagnosis. (C) 2004 Elsevier B.V. All rights reserved.