Comparison of Sebia Capillarys Capillary Electrophoresis With the Primus High-Pressure Liquid Chromatography in the Evaluation of Hemoglobinopathies
Comparison of Sebia Capillarys Capillary Electrophoresis With the Primus High-Pressure Liquid Chromatography in the Evaluation of Hemoglobinopathies
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DOI：
10.1309/ajcpqy80hzwhhgzf
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发表时间：
2008-11-01
影响因子：
3.5
通讯作者：
Bak, Richard
中科院分区：
文献类型：
--
作者：
Keren, David F.;Hedstrom, Deborah;Bak, Richard
Sebia Capillarys（毛细管区带电泳[CE]）和Primus Resolution高压液相色谱（HPLC）用于前瞻性评价297份血红蛋白病样本。两种技术的血红蛋白（Hb）A水平相似（CE法平均值为96.2%，SD为5.7%; HPLC法平均值为96.8%，SD为5.5%），但CE法HbA（2）水平（平均值为2.8%; SD为0.8%）高于HPLC法（平均值为2.3%; SD为0.8%）。CE法测得的HbS值（平均值，40.6%; SD，18.9%）高于HPLC法测得的值（平均值，38.4%; SD，18.9%）。在Hg S患者中，HPLC法测得的HbA（2）水平（平均值4.0%;标准差1.0%）高于CE法测得的HbA（2）水平（平均值3.1%;标准差0.8%）。HbA（2）偶尔不能与HbC充分分离，无法通过CE进行测量，但可以通过CE与HbE分离。两种方法一起鉴定了HbS、HbC、HbE、HbS和HbC，HbA α 2 β、HbD-洛杉矶、HbF变体、HbG-费城、HbS-G费城和Hb Lepore。
The Sebia Capillarys (capillary zone electrophoresis [CE]) and the Primus Resolution high-pressure liquid chromatography (HPLC) were used to prospectively evaluate 297 samples for hemoglobinopathies. Hemoglobin (Hb) A levels were similar on both techniques (mean, 96.2% and SD, 5.7% by CE; mean, 96.8% and SD, 5.5% by HPLC), but HbA(2) levels were higher by CE (mean, 2.8%; SD, 0.8%) than by HPLC (mean, 2.3%; SD, 0.8%). HbS had higher values by CE (mean, 40.6%; SD, 18.9%) than by HPLC (mean, 38.4%; SD, 18.9%). In cases with Hg S, HbA(2) levels were greater by HPLC (mean, 4.0%; SD, 1.0%) than by CE (mean, 3.1%; SD, 0.8%). HbA(2) was occasionally not separated sufficiently from HbC for measurement by CE, but did separate from HbE by CE. Both methods identified HbS, HbC, HbE, HbS, and HbC together, HbA(2)', HbD-Los Angeles, HbF variant, HbG-Philadelphia, HbS-G Philadelphia, and Hb Lepore.