SAGE library screening reveals ILT7 as a specific plasmacytoid dendritic cell marker that regulates type IIFN production
SAGE library screening reveals ILT7 as a specific plasmacytoid dendritic cell marker that regulates type IIFN production
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DOI：
10.1093/intimm/dxm127
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发表时间：
2008-01-01
影响因子：
4.4
通讯作者：
Kamogawa-Schifter, Yumiko
中科院分区：
文献类型：
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作者：
Cho, Minkwon;Ishida, Koji;Kamogawa-Schifter, Yumiko
浆细胞样树突状细胞（pDCs）通过在感染时产生高水平的I型IFN，将先天免疫反应与获得性免疫反应联系起来。为了确定控制pDC的特异性基因，我们比较了人类pDC、单纯疱疹病毒刺激的pDC和单核细胞的基因表达文库的序列分析。我们发现igg样转录物ILT7在pDC细胞表面特异性表达，当pDC在病毒或细菌刺激下成熟时，ILT7被下调。细胞表面的ILT7表达需要与Fc epsilon RI γ接头分子相关联。尽管一种抗ilt7特异性单抗在细胞嘧啶-磷酸-鸟苷（CpG）刺激下抑制了I型IFN的产生，但另一种抗ilt7单抗上调了I型IFN的产生。我们得出结论，ILT7是人类pDC功能的关键调节因子。
Plasmacytoid dendritic cells (pDCs) link innate to acquired immune responses by producing high levels of type I IFN upon infection. In order to identify the specific genes that control pDC, we compared serial analysis of gene expression libraries from human pDCs, herpes simplex virus-stimulated pDCs and monocytes. We found that Ig-like transcript ILT7 is specifically expressed on pDC cell surfaces and is down-regulated when pDC mature in response to viral or bacterial stimulation. ILT7 expression on the cell surface required association with the Fc epsilon RI gamma adaptor molecule. Although treatment with one anti-ILT7-specific mAb suppressed type I IFN production in response to cytosine-phosphate-guanosice (CpG) stimulation, another anti-ILT7 mAb up-regulated type I IFN production. We conclude that ILT7 is a key regulator of human pDC function.