Disordered array of Au covered Silicon nanowires for SERS biosensing combined with electrochemical detection.
Disordered array of Au covered Silicon nanowires for SERS biosensing combined with electrochemical detection.
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DOI：
10.1038/srep25099
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发表时间：
2016-04-26
影响因子：
4.6
通讯作者：
Maiolo L
中科院分区：
文献类型：
--
作者：
Convertino A;Mussi V;Maiolo L
我们报道了高度无序排列的镀金硅纳米线（Au/SiNWs）作为表面增强拉曼散射（SERS）探针与电化学检测相结合的生物传感应用。采用等离子体增强化学气相沉积法在普通显微镜载玻片上生长了几微米长的SiNWs，并覆盖了厚度为150 nm的Au蒸发膜。通过生物素-亲和素相互作用研究了所得到的复合结构作为SERS生物传感器的能力：从该结构获得的拉曼信号允许遵循每个表面修饰步骤，以及在从微摩尔到纳摩尔值的广泛浓度范围内有效检测亲和素分子。利用包裹SiNWs的金属覆盖物，利用电化学阻抗谱（EIS）实现了对同一生物分析物的双重检测。实际上，在同样的三电极装置上，用Au/SiNWs作为工作电极和SERS探针，监测了生物分子扰动在NWs金属化表面引起的SERS信号和阻抗变化。
We report on highly disordered array of Au coated silicon nanowires (Au/SiNWs) as surface enhanced Raman scattering (SERS) probe combined with electrochemical detection for biosensing applications. SiNWs, few microns long, were grown by plasma enhanced chemical vapor deposition on common microscope slides and covered by Au evaporated film, 150 nm thick. The capability of the resulting composite structure to act as SERS biosensor was studied via the biotin-avidin interaction: the Raman signal obtained from this structure allowed to follow each surface modification step as well as to detect efficiently avidin molecules over a broad range of concentrations from micromolar down to the nanomolar values. The metallic coverage wrapping SiNWs was exploited also to obtain a dual detection of the same bioanalyte by electrochemical impedance spectroscopy (EIS). Indeed, the SERS signal and impedance modifications induced by the biomolecule perturbations on the metalized surface of the NWs were monitored on the very same three-electrode device with the Au/SiNWs acting as both working electrode and SERS probe.