Geminivirus DNA replication and cell cycle interactions
Geminivirus DNA replication and cell cycle interactions
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DOI：
10.1016/j.vetmic.2003.10.012
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发表时间：
2004-02-04
影响因子：
3.3
通讯作者：
Missich, R
中科院分区：
文献类型：
--
作者：
Gutierrez, C;Ramirez-Parra, E;Missich, R
双生病毒科包括大量侵染植物的病毒，具有独特的双生病毒粒子，即类似2.6-3.0kb的单链基因组，并通过滚环机制进行复制。由于它们只编码几种蛋白质(4-6种取决于属于四个不同属的成员)，病毒蛋白质和宿主因子之间的丰富多样的相互作用已经演变成病毒复制周期。其中，到目前为止，我们特别感兴趣的是：(I)对视网膜母细胞瘤相关(RBR)/E2F途径的细胞周期调节蛋白的干扰；(Ii)与宿主DNA复制因子的相互作用，这是在DNA复制的病毒起始处在滚环阶段组装功能复制复合体所必需的。酵母双杂交结果表明，小麦矮缩病毒REPA蛋白与植物RBR蛋白相互作用，但不与植物RBR蛋白相互作用。有趣的是，Rep的C-末端结构域的缺失使截短的蛋白能够与RBR相互作用，这表明该结构域可能阻碍了LXCXE RBR结合基序。二级结构预测支持这种可能性。(C)2003爱思唯尔B.V.保留所有权利。
The Geminiviridae family includes a large number of viruses that infect plants and have a unique geminate virion particle, a single-stranded genome of similar to2.6-3.0 kb, and replicate through a rolling-circle mechanism. Since they encode for just a few proteins (4-6 depending on the members that belong to four different genera), a rich variety of interactions has evolved between viral proteins and host factors to develop the virus replicative cycle. Among them, we have been particularly interested so far: (i) in the interference with cell cycle regulatory proteins of the retinoblastoma-related (RBR)/E2F pathway and (ii) in the interaction with host DNA replication factors necessary for the assembly of a functional replication complex at the viral origin of DNA replication during the rolling-circle stage. Yeast two-hybrid assays revealed that wheat dwarf virus RepA protein, but nor Rep protein, interacts with plant RBR protein. Interestingly, deletion of the C-terminal domain of Rep confers the truncated protein the ability to interact with RBR, suggesting that this domain may hinder the LXCXE RBR-binding motif. Secondary structure predictions support such a possibility. (C) 2003 Elsevier B.V. All rights reserved.