Two-Photon Optogenetic Stimulation of Drosophila Neurons.
Two-Photon Optogenetic Stimulation of Drosophila Neurons.
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果蝇神经元的双光子光遗传学刺激。
DOI：
10.1007/978-1-0716-0830-2_7
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Jeanne,JamesM
中科院分区：
文献类型：
--
作者：
Fişek,Mehmet;Jeanne,JamesM
光遗传学能够利用光对神经活动进行实验控制。视紫红质及其变体通常使用可见光激发来激活，但也可以使用红外双光子激发来激活。双光子激发可以提高散射组织中刺激的空间精度，但在使用前需要考虑几个实际限制。在这里，我们描述的方法和最佳实践，使用双光子光遗传学刺激的神经元内的果蝇，果蝇的大脑，重点是触角叶的投射神经元。
Optogenetics enables experimental control over neural activity using light. Channelrhodopsin and its variants are typically activated using visible light excitation but can also be activated using infrared two-photon excitation. Two-photon excitation can improve the spatial precision of stimulation in scattering tissue but has several practical limitations that need to be considered before use. Here we describe the methodology and best practices for using two-photon optogenetic stimulation of neurons within the brain of the fruit fly,Drosophila melanogaster, with an emphasis on projection neurons of the antennal lobe.