A microfluidic device for electrofusion of biological vesicles
A microfluidic device for electrofusion of biological vesicles
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DOI：
10.1023/b:bmmd.0000042050.95246.af
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发表时间：
2004-09-01
影响因子：
2.8
通讯作者：
Takeuchi, S
中科院分区：
文献类型：
--
作者：
Tresset, G;Takeuchi, S
本文报道了一种用于脂质体与细胞电融合的具有高深宽比电极和低功耗的微加工装置。其应用范围可以从基因转染或细胞跟踪到膜蛋白的生物物理研究。该器件由250 μ m厚的硅电极粘合到玻璃基板上，并由PDMS涂覆的载玻片覆盖。脂质体首先通过300 kHz的AC电压排列，然后用短DC脉冲融合。融合产率可以达到75%，并且对于大于10 μ m的脂质体直径总体上更好。微珠在脂质体内的包封也已得到证实，并开辟了将人造微结构基于融合递送到细胞中的途径。
This paper reports a microfabricated device with high aspect-ratio electrodes and low power consumption for the electrofusion of liposomes and cells. The applications may range from gene transfection or cell tracking to biophysical studies of membrane proteins. The device consists of 250 mum thick silicon electrodes bonded to a glass substrate and covered by a PDMS-coated glass slide. Liposomes were first aligned by AC voltage at 300 kHz and then fused with short DC pulses. The fusion yield can reach 75% and is globally better for liposome diameters larger than 10 pm. The encapsulation of microbeads inside liposomes has also been demonstrated and opens up the route towards fusion-based delivery of artificial microstructures into cells.