cDNA cloning and transcriptional regulation of the vitellogenin receptor from the imported fire ant, Solenopsis invicta Buren (Hymenoptera: Formicidae)
cDNA cloning and transcriptional regulation of the vitellogenin receptor from the imported fire ant, Solenopsis invicta Buren (Hymenoptera: Formicidae)
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DOI：
10.1111/j.0962-1075.2004.00477.x
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发表时间：
2004-04-01
影响因子：
2.6
通讯作者：
Pietrantonio, PV
中科院分区：
文献类型：
--
作者：
Chen, ME;Lewis, DK;Pietrantonio, PV
报道了从外来入侵害虫红火蚁（Solenopsis invicta）中克隆的首个膜翅目卵黄蛋白原受体（VgR） cDNA。利用逆转录聚合酶链反应和cDNA末端的快速扩增，克隆了包含整个VgR编码区的片段并对其进行了测序。全长5764bp的cDNA编码1782残基蛋白，预测分子量为201.3 kDa （=SiVgR）。Northern blot分析表明，7.4 kb的SiVgR转录本仅存在于生殖雌性（未交配的雄性和雌性）的卵巢中。转录表达的时间谱显示，SiVgR mRNA随着年龄的增长而增加，这是由甲基戊二烯，一种幼崽激素（JH）类似物上调。这表明SiVgR基因受JH调控。
We describe the cloning of the first hymenopteran vitellogenin receptor (VgR) cDNA from the imported fire ant, Solenopsis invicta, an invasive pest. Using reverse transcription polymerase chain reaction and rapid amplification of cDNA ends, fragments encompassing the entire coding region of a putative VgR were cloned and sequenced. The complete 5764 bp cDNA encodes a 1782 residue protein with a predicted molecular mass of 201.3 kDa (=SiVgR). Northern blot analysis demonstrated that the 7.4 kb SiVgR transcript was present only in ovaries of reproductive females (virgin alates and queens). The temporal profile of transcriptional expression showed that SiVgR mRNA increased with age in virgin alate females and that this was up-regulated by methoprene, a juvenile hormone (JH) analogue. This suggests that the SiVgR gene is JH regulated.