EFFECT OF VASOACTIVE-INTESTINAL-PEPTIDE ON MONKEY PROLACTIN SECRETION AND CYCLIC-AMP IN CULTURE - INTERACTION WITH ESTRADIOL AND PHENOL RED
EFFECT OF VASOACTIVE-INTESTINAL-PEPTIDE ON MONKEY PROLACTIN SECRETION AND CYCLIC-AMP IN CULTURE - INTERACTION WITH ESTRADIOL AND PHENOL RED
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DOI：
10.1159/000125394
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发表时间：
1990-05-01
影响因子：
4.1
通讯作者：
BETHEA, CL
中科院分区：
文献类型：
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作者：
BETHEA, CL
为了检验雌激素化合物可能降低灵长类催乳素对腺苷酸环化酶介导的促分泌素的敏感性的假设，在存在和不存在雌二醇 (E) 和酚红的情况下，检查了 VIP 对无血清猴垂体单层培养物中催乳素分泌和 cAMP 水平的影响。在两个实验设计中，E 处理在分散后的第二天（分体板设计）或无血清培养后 10 天（全板设计）开始。在培养第 10 天和第 20 天，进行 VIP 挑战（5、50 和 500 nM）4 小时。当用 E 或酚红处理培养物时，VIP 对催乳素的最大刺激百分比显着降低。在没有酚红的情况下，5、50 和 500 nM VIP 催乳素的平均增加百分比分别为 23%、83% 和 156%，但当向不含酚红的培养物中添加 E 时，催乳素的平均百分比增加分别为 14%、43% 和 112%。在酚红存在下，VIP 的刺激百分比平均为 32%、62% 和 97%，但添加 E 和酚红后，平均刺激百分比分别降低至 26%、45% 和 72%。 E 和酚红增加了 cAMP 的基础水平。然而，在 E 和酚红存在下，VIP 对 cAMP 的最大刺激百分比降低。总之，E 和酚红可降低 VIP 对催乳素分泌的最大刺激百分比。这种效应通过细胞内 cAMP 最大刺激百分比的降低来反映。
To test the hypothesis that estrogenic compounds may decrease the sensitivity of primate lactotropes to adenylate cyclase-mediated secretagogues, the effect of VIP on prolactin secretion and cAMP levels in serum-free monkey pituitary monolayer cultures was examined in the presence and absence of estradiol (E) and phenol red. In two experimental designs, E treatment was initiated on either the day after dispersion (split plate design) or 10 days after serum-free culture (whole plate design). VIP challenges (5, 50 and 500 nM) were administered for 4 h on days 10 and 20 of culture. There was a significant decrease in the maximal percent stimulation of prolactin by VIP when cultures were treated with E or phenol red. The average percent increase in prolactin at 5, 50 and 500 nM VIP equalled 23, 83 and 156% in the absence of phenol red, but equalled 14, 43 and 112% when E was added to phenol red-free cultures. The percent stimulation by VIP in the presence of phenol red averaged 32, 62 and 97%, but addition of E with phenol red decreased the average stimulation to 26, 45 and 72%, respectively. Basal levels of cAMP were increased by E and phenol red. However, the maximal percent stimulation of cAMP by VIP was decreased in the presence of E and phenol red. In summary, E and phenol red act to decrease the maximal percent stimulation of prolactin secretion by VIP. This effect is reflected by a decrease in the maximal percent stimulation of intracellular cAMP.