Olfactory projectome in the zebrafish forebrain revealed by genetic single-neuron labelling
Olfactory projectome in the zebrafish forebrain revealed by genetic single-neuron labelling
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DOI：
10.1038/ncomms4639
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发表时间：
2014-04-01
影响因子：
16.6
通讯作者：
Yoshihara, Yoshihiro
中科院分区：
文献类型：
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作者：
Miyasaka, Nobuhiko;Arganda-Carreras, Ignacio;Yoshihara, Yoshihiro
嗅球中的化学定位气味表征被转移到多个前脑区域，并转化为适当的输出响应。然而，一个全面的投射地图延髓输出神经元在单轴突分辨率是缺乏脊椎动物。在这里，我们通过遗传单神经元追踪和图像配准解开了斑马鱼嗅球的投影图。我们发现，五个主要的目标区域收到不同的模式，从嗅球肾小球的预测。端脑后部的中央部分接收来自所有肾小球的非选择性的、散布的输入，而端脑腹侧由来自特定肾小球簇的轴突弥漫性支配。右侧缰核和后结节（间脑核）分别接受来自限制性和所有肾小球簇的会聚输入。延髓的复发性投射是粗糙的地形。因此，初级的趋化性组织在高级嗅觉中心转化为不同的感觉表征。这些发现提供了一个框架，以了解一般原则，以及物种特异性功能解码的气味信息。
Chemotopic odour representations in the olfactory bulb are transferred to multiple forebrain areas and translated into appropriate output responses. However, a comprehensive projection map of bulbar output neurons at single-axon resolution is lacking in vertebrates. Here we unravel a projectome of the zebrafish olfactory bulb through genetic single-neuron tracing and image registration. We show that five major target regions receive distinct modes of projections from olfactory bulb glomeruli. The central portion of posterior telencephalon receives non-selective, interspersed inputs from all glomeruli, whereas the ventral telencephalon is diffusely innervated by axons from particular glomerular clusters. The right habenula and posterior tuberculum (diencephalic nuclei) receive convergent inputs from restricted and all glomerular clusters, respectively. The bulbar recurrent projections are coarsely topographic. Thus, the primary chemotopic organization is transformed into distinct sensory representations in higher olfactory centres. These findings provide a framework to understand general principles as well as species-specific features in decoding of odour information.