Starter Feeding Supplementation Alters Colonic Mucosal Bacterial Communities and Modulates Mucosal Immune Homeostasis in Newborn Lambs.
Starter Feeding Supplementation Alters Colonic Mucosal Bacterial Communities and Modulates Mucosal Immune Homeostasis in Newborn Lambs.
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DOI：
10.3389/fmicb.2017.00429
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发表时间：
2017
影响因子：
5.2
通讯作者：
Mao S
中科院分区：
文献类型：
--
作者：
Liu J;Bian G;Sun D;Zhu W;Mao S
本研究旨在探讨日粮中添加开食料对断奶前羔羊结肠黏膜细菌群落和黏膜免疫稳态的影响。我们选择了8对10天大的羔羊双胞胎。一对双胞胎喂母乳（M，n = 8），而另一个喂母乳加起动机（M+S，n = 8）。在56日龄时处死羔羊。收集结肠内容物以测定pH以及挥发性脂肪酸（VFA）和乳酸盐的浓度。收获结肠粘膜以使用Illumina MiSeq测序表征细菌群落，并使用定量实时PCR确定细胞因子和toll样受体（TLR）的mRNA表达水平。结果表明，发酵剂喂养降低了管腔pH值，并增加了结肠中乙酸盐、丙酸盐、丁酸盐、总VFA和乳酸盐的浓度。主坐标分析（PCA）和分子方差分析表明，添加发酵剂显著影响了结肠粘膜细菌群落，其中优势类群未分类的S24-7、颤杆菌属、普雷沃氏菌属、副拟杆菌属、双歧杆菌属、反刍兽杆菌属和琥珀酸弧菌属的相对丰度较高，未分类的瘤胃球菌科、RC9_gut_group、布劳特氏菌属、Phocaeicola、Phascolarctobacterium、未分类的BS11_gut_group、未分类的family_XIII和羔羊中的弯曲杆菌。同时，预饲降低结肠组织中TLR 4和细胞因子TNF-α、IFN-γ mRNA的表达。此外，结肠粘膜TLR和细胞因子mRNA表达的变化与粘膜细菌组成的变化相关。这些发现可能为了解羔羊结肠粘膜细菌和免疫稳态提供新的见解。
This study aims to investigate the effect of starter feeding supplementation on colonic mucosal bacterial communities and on mucosal immune homeostasis in pre-weaned lambs. We selected eight pairs of 10-day-old lamb twins. One twin was fed breast milk (M, n = 8), while the other was fed breast milk plus starter (M+S, n = 8). The lambs were sacrificed at 56 days age. Colonic content was collected to determine the pH and the concentrations of volatile fatty acids (VFA) and lactate. The colonic mucosa was harvested to characterize the bacterial communities using Illumina MiSeq sequencing and to determine mRNA expression levels of cytokines and toll-like receptors (TLR) using quantitative real-time PCR. The results show that starter feeding decreased luminal pH and increased the concentrations of acetate, propionate, butyrate, total VFA, and lactate in the colon. The principal coordinate analysis (PCA) and analysis of molecular variance show that starter feeding supplementation significantly affected the colonic mucosal bacterial communities with a higher relative abundance of the dominant taxa unclassified S24-7, Oscillibacter, Prevotella, Parabacteroides, Bifidobacterium, Ruminobacter, and Succinivibrio, and a lower proportion of unclassified Ruminococcaceae, RC9_gut_group, Blautia, Phocaeicola, Phascolarctobacterium, unclassified BS11_gut_group, unclassified family_XIII, and Campylobacter in lambs. Meanwhile, starter feeding decreased mRNA expression of TLR4 and cytokines TNF-α and IFN-γ in colonic tissue. Furthermore, the changes in the colonic mucosal mRNA expression of TLR and cytokines were associated with changes in mucosal bacterial composition. These findings may provide new insights into colonic mucosal bacteria and immune homeostasis in developing lambs.