ATP Binding Cassette Transporter ABCA7 Regulates NKT Cell Development and Function by Controlling CD1d Expression and Lipid Raft Content.
ATP Binding Cassette Transporter ABCA7 Regulates NKT Cell Development and Function by Controlling CD1d Expression and Lipid Raft Content.
复制标题
DOI：
10.1038/srep40273
复制
发表时间：
2017-01-16
影响因子：
4.6
通讯作者：
Hedrick CC
中科院分区：
文献类型：
--
作者：
Nowyhed HN;Chandra S;Kiosses W;Marcovecchio P;Andary F;Zhao M;Fitzgerald ML;Kronenberg M;Hedrick CC
ABCA 7是一种在质膜上表达的ABC转运蛋白，并将磷脂复合物从细胞质主动输出到膜的细胞外质小叶。不变NKT（iNKT）细胞是T淋巴细胞的亚群，其在CD 1d介导的抗原呈递的背景下识别糖脂抗原。在这项研究中，我们证明了ABCA 7以细胞外的方式调节NKT细胞的发育。我们发现，在Abca 7 −/−小鼠中，CD 1d的表达减少，伴随着胸腺细胞和抗原呈递细胞质膜上脂筏含量的改变。总之，这些由ABCA 7缺乏引起的改变对NKT细胞的发育和功能产生负面影响。
ABCA7 is an ABC transporter expressed on the plasma membrane, and actively exports phospholipid complexes from the cytoplasmic to the exocytoplasmic leaflet of membranes. Invariant NKT (iNKT) cells are a subpopulation of T lymphocytes that recognize glycolipid antigens in the context of CD1d-mediated antigen presentation. In this study, we demonstrate that ABCA7 regulates the development of NKT cells in a cell-extrinsic manner. We found that in Abca7−/− mice there is reduced expression of CD1d accompanied by an alteration in lipid raft content on the plasma membrane of thymocytes and antigen presenting cells. Together, these alterations caused by absence of ABCA7 negatively affect NKT cell development and function.