Evaluation of the RIDA®QUICK immunochromatographic norovirus detection assay using specimens from Australian gastroenteritis incidents
Evaluation of the RIDA®QUICK immunochromatographic norovirus detection assay using specimens from Australian gastroenteritis incidents
复制标题
DOI：
10.1016/j.jviromet.2011.01.017
复制
发表时间：
2011-04-01
影响因子：
3.1
通讯作者：
Marshall, John A.
中科院分区：
文献类型：
--
作者：
Bruggink, Leesa D.;Witlox, Kristie J.;Marshall, John A.
诺沃克病毒是引致肠胃炎的主要原因，现时有一系列化验方法可供检测。最近，一种用于诺如病毒检测的商业免疫层析测定法，RIDA（R）QUICK测定法，已经变得可用，但是关于其功效的信息仍然有限。本研究使用澳大利亚一家主要诊断/参考实验室检测的粪便材料，检查了RIDA（R）QUICK检测试剂盒的灵敏度和特异性。该方法的敏感性为83%，特异性为100%。未发现诺如病毒假阳性结果，该检测试剂盒与常见粪便病毒（如轮状病毒、星状病毒、痘病毒和腺病毒）无交叉反应。基因组I（GI）诺如病毒的检测可靠性低于基因组II（GII）诺如病毒。该检测试剂盒检测到的基因型包括GII.1、GII.2、GII.3、GII.4、GII.6和GII.7。该检测试剂盒未检出试验组中的任何GI标本。未检测到的基因型包括GI. 4和GI. 6。该测定方法简单、快速。它在需要快速初始诺如病毒结果的护理点情况下或作为实验室的备份是有价值的。(C)2011爱思唯尔有限公司版权所有。
A range of laboratory methods is now available for the detection of norovirus, a major cause of gastroenteritis. Recently, a commercial immunochromatographic assay for norovirus detection, the RIDA (R) QUICK assay, has become available, but there is still only limited information on its efficacy. This study examined the sensitivity and specificity of the RIDA (R) QUICK assay, using faecal material received for testing in a major diagnostic/reference laboratory in Australia. The sensitivity of the assay was found to be 83% and the specificity was 100%. No false positive norovirus results were found and the assay did not cross-react with common faecal viruses such as rotavirus, astrovirus, sapovirus and adenovirus. The assay was less reliable for genogroup I (GI) noroviruses than for genogroup II (GII) noroviruses. Genotypes detected by the assay included GII.1, GII.2, GII.3, GII.4, GII.6 and GII.7. The assay failed to detect any GI specimens in the test group. Genotypes not detected included GI.4 and GI.6. The assay was simple and quick to perform. It is valuable in a point-of-care situation or as a backup in a laboratory where a rapid initial norovirus result is required. (C) 2011 Elsevier B.V. All rights reserved.