Synthesis of eosinophil-associated enzymes in HL-60 promyelocytic leukemia cells.
Synthesis of eosinophil-associated enzymes in HL-60 promyelocytic leukemia cells.
复制标题
HL-60 早幼粒细胞白血病细胞中嗜酸性粒细胞相关酶的合成。
作者：
S. Fischkoff;G. E. Brown;A. Pollak
来源于HL-60细胞的嗜酸性粒细胞与某些恶性肿瘤患者的嗜酸性粒细胞具有许多常见的颗粒组织化学和形态异常，特别是在伴有异常嗜酸性粒细胞的急性髓-单核细胞白血病(FAB-M4eo)中。为了了解这些异常的发病机制，我们研究了HL-60早幼粒白血病细胞在嗜酸性粒细胞分化的不同阶段所特有的四种酶。利用生化和超组织化学技术，证实了与正常嗜酸性粒细胞发育的以下不同之处。首先，髓过氧化物酶和嗜酸性粒细胞过氧化物酶在成熟的HL-60嗜酸性粒细胞群体中共存。其次，颗粒是由内质网扩张段产生的液泡中的物质凝聚而成的；高尔基体在处理过氧化物酶过程中的作用似乎很小。第三，与正常的嗜酸性粒细胞相比，细胞中的溶血磷脂酶和芳基硫酸酯酶水平较低。最后，在约5%的细胞颗粒中出现了类似于Auer棒前体结构的结晶。这些结果提示，HL-60嗜酸性粒细胞存在蛋白质合成和加工的调控障碍，这可能是其颗粒形态和组织化学异常的原因之一。
Eosinophils derived from HL-60 cells share many of the abnormalities of granule histochemistry and morphology frequently seen in eosinophils of patients with certain malignancies, especially those seen in acute myelomonocytic leukemia with abnormal eosinophils (FAB class M4eo). In order to understand the pathogenesis of these abnormalities, four enzymes, characteristic of the eosinophil, were studied in HL-60 promyelocytic leukemia cells at various stages of eosinophilic differentiation. Using biochemical and ultrahistochemical techniques, the following differences from normal eosinophil development were demonstrated. First, both myeloperoxidase and eosinophil peroxidase coexisted in the population of maturing HL-60 eosinophils. Second, the granules formed from the condensation of material in vacuoles which were derived from dilated segments of the endoplasmic reticulum; the role of the Golgi apparatus in processing of peroxidase appeared minimal. Third, low levels of lysophospholipase and arylsulfatase were present in the cells compared to normal eosinophils. Finally, crystallizations resembling precursor structures of Auer rods appeared in the granules of about 5% of the cells. These findings suggest that several disorders of the control of protein synthesis and processing exist in HL-60 eosinophils which may be responsible for the abnormal granule morphology and histochemistry.
嗜酸性白细胞溶酶体酸性磷酸酶的激活。
DOI：
--
发表时间：
1981
期刊：
Laboratory investigation; a journal of technical methods and pathology
影响因子：
--
作者：
Bass,DA;Lewis,JC;Szejda,P;Cowley,L;McCall,CE
通讯作者：
McCall,CE
影响因子：
20.3
作者：
H. Koeffler;J. Ranyard;M. Pertcheck
通讯作者：
H. Koeffler;J. Ranyard;M. Pertcheck