Prevalence of Yersinia enterocolitica and Yersinia pseudotuberculosis in wild boars in the Basque Country, northern Spain
Prevalence of Yersinia enterocolitica and Yersinia pseudotuberculosis in wild boars in the Basque Country, northern Spain
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DOI：
10.1186/s13028-016-0184-9
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发表时间：
2016-01-20
影响因子：
2.1
通讯作者：
Barral, Marta
中科院分区：
文献类型：
--
作者：
Arrausi-Subiza, Maialen;Gerrikagoitia, Xeider;Barral, Marta
背景：耶尔森菌病是一种广泛分布于欧洲的人畜共患病，猪携带不同血清型的小肠结肠炎耶尔森菌和假结核耶尔森菌。本研究的目的是确定西班牙北部野猪肠结肠炎和假结核耶氏菌的流行情况。在2001年至2012年期间采集了505只野猪的血液样本。用间接酶联免疫吸附法测定490份血清样本的血清阳性率。用实时聚合酶链反应检测72只动物扁桃体中是否存在小肠结肠炎或假结核杆菌。所有扁桃体直接和在添加1%甘露醇和0.15%胆盐的磷酸盐缓冲盐水中冷富集后进行两次分析。结果：52.5%的动物检出小肠结肠炎耶夫氏菌和假结核耶夫氏菌抗体。实时聚合酶链反应检测出小肠结肠炎耶尔森菌33.3%，假结核耶尔森菌25%。不同采样年份差异显著，冬季和春季患病率最高。在海拔600 m以上的山区，抗体水平和小肠结肠炎患病率最高，冬季非常寒冷，各优势气候的年降雨量最高。畜禽种群数量中低的地区野猪血清耶尔森氏菌感染率最高，而绵羊种群数量高的地区小肠结肠炎耶尔森氏菌感染率最高。结论：本研究表明巴斯克地区野猪中小肠结肠炎耶氏菌和假结核耶氏菌高度流行，其中以小肠结肠炎耶氏菌最为流行。野猪感染的风险受季节和它们生活的地区的影响。
Background: Yersiniosis is a zoonosis widely distributed in Europe and swine carry different serotypes of Yersinia enterocolitica and Y. pseudotuberculosis. The aim of this study was to determine the prevalence of Y. enterocolitica and Y. pseudotuberculosis in wild boars in northern Spain. The blood of wild boars (n = 505) was sampled between 2001 and 2012. Seroprevalence was determined in 490 serum samples with an indirect enzyme-linked immunosorbent assay. Seventy-two of the animals were also examined for the presence of Y. enterocolitica or Y. pseudotuberculosis in the tonsils with real-time polymerase chain reaction. All the tonsils were analysed twice, directly and after cold enrichment in phosphate-buffered saline supplemented with 1 % mannitol and 0.15 % bile salts.Results: Antibodies directed against Y. enterocolitica and Y. pseudotuberculosis were detected in 52.5 % of the animals. Yersinia enterocolitica was detected with real-time polymerase chain reaction in 33.3 % of the wild boars and Y. pseudotuberculosis in 25 %. Significant differences were observed according to the sampling year, and the highest prevalence was during winter and spring. The highest antibody levels and Y. enterocolitica prevalence were observed in mountainous areas at altitudes higher than 600 m, with very cold winters, and with the highest annual rainfall for each dominant climate. Areas with low and medium livestock populations were associated with the highest seroprevalence of Yersinia spp. in wild boars, whereas areas with high ovine populations had the highest prevalence of Y. enterocolitica.Conclusions: This study shows that Y. enterocolitica and Y. pseudotuberculosis are highly prevalent among wild boars in the Basque country, with Y. enterocolitica most prevalent. The risk of infection among wild boars is influenced by the season and the area in which they live.