DNA BENDING AND ORIENTATION-DEPENDENT FUNCTION OF YY1 IN THE C-FOS PROMOTER
DNA BENDING AND ORIENTATION-DEPENDENT FUNCTION OF YY1 IN THE C-FOS PROMOTER
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DOI：
10.1101/gad.7.12b.2497
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发表时间：
1993-12-01
影响因子：
10.5
通讯作者：
GILMAN, MZ
中科院分区：
文献类型：
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作者：
NATESAN, S;GILMAN, MZ
在启动子、增强子和沉默子处组装多组分复合物可能需要DNA螺旋路径的扰动。我们目前的证据表明，YY 1，一个普遍表达的DNA结合蛋白，调节c-fos启动子的活性主要是通过对DNA结构的影响。YY 1结合并诱导该启动子中三个位点的相控DNA弯曲。通过使用一个截短的c-fos启动子活性含有一个单一的功能YY 1网站，我们表明，YY 1抑制启动子活性，但在这种情况下，抑制似乎不是一个内在的属性的蛋白质。此外，当YY 1位点的方向反转时，YY 1激活相同的启动子。通过改变YY 1两侧因子结合位点的相对相位也可以减轻YY 1的抑制作用。我们的结论是，YY 1在这个启动子的主要功能是弯曲DNA调节其他蛋白质之间的接触。因此，YY 1代表一类新的转录因子，其通过影响启动子结构而不是直接接触转录机器来影响启动子功能。我们提供的证据表明，产品的男性性别决定基因SRY也可能属于这一类的结构因素。
The assembly of multicomponent complexes at promoters, enhancers, and silencers likely entails perturbations in the path of the DNA helix. We present evidence that YY1, a ubiquitously expressed DNA-binding protein, regulates the activity of the c-fos promoter primarily through an effect on DNA structure. YY1 binds to and induces a phased DNA bend at three sites in this promoter. By use of a truncated c-fos promoter activity containing a single functional YY1 site, we show that YY1 represses promoter activity but that repression does not appear to be an intrinsic property of the protein in this context. Moreover, when the orientation of the YY1 site is reversed, YY1 activates the same promoter. Repression by YY1 is also alleviated by changing the relative phasing of factor-binding sites on either side of YY1. We conclude that the principal function of YY1 in this promoter is to bend DNA to regulate contact between other proteins. Thus, YY1 represents a new class of transcription factors that influences promoter function by affecting promoter structure rather than by directly contacting the transcriptional machinery. We provide evidence that the product of the male sex determination gene SRY may also belong to this class of structural factors.