SPINOTHALAMOCORTICAL PROJECTIONS TO THE SECONDARY SOMATOSENSORY CORTEX (SII) IN SQUIRREL-MONKEY
SPINOTHALAMOCORTICAL PROJECTIONS TO THE SECONDARY SOMATOSENSORY CORTEX (SII) IN SQUIRREL-MONKEY
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DOI：
10.1016/0006-8993(93)91541-y
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发表时间：
1993-12-24
期刊：
影响因子：
2.9
通讯作者：
APKARIAN, AV
中科院分区：
文献类型：
--
作者：
STEVENS, RT;LONDON, SM;APKARIAN, AV
颈脊髓丘脑束的顺行标记与投射到第二体感皮层（hSII）的手区域的丘脑皮质细胞的逆行标记相结合，以确定丘脑中可能的位点，用于处理和传递伤害性信息到hSII。顺行标记的终端进行了2%的WGA-HRP注射在颈部扩大;丘脑皮质细胞逆行标记荧光示踪剂。在一个实验中，注射对侧的初级体感皮质手区（hSI），以提供与hSII丘脑标记的直接比较。标记细胞和终末样结构均在单个丘脑切片中可视化，并对其数量和位置进行定量分析。将距离STT末端100微米内的标记细胞数计数为重叠细胞。丘脑腹后下核（VPI）、腹后外侧核（VPI）、后核（PO）和中央外侧核（CL）共包含86.5%的hSII投射重叠细胞。在所有投射hSII的丘脑重叠细胞中，VPI所含的数量最多（占总数的36.4%），其次是后核复合体的前部（POa; 20.4%），VPL（18.3%）和CL（11.4%）。hSI注射的结果显示与我们早期研究一致的不同重叠模式。重叠细胞的相对分布依赖于SII注射的前后位置。最前面的注射导致少量的标记细胞，大多数重叠的细胞位于PO和CL。后壁注射次数越多，重叠细胞主要出现在VPI和VPL。结果表明，在松鼠猴中，VPI、VPL、POa和CL将伤害性信息从脊髓传递到第二躯体感觉皮层。
Anterograde labeling of the cervical spinothalamic tract was combined with retrograde labeling of thalamocortical cells projecting to the hand region of the second somatosensory cortex (hSII) to identify likely sites in the thalamus for processing and transmitting nociceptive information to hSII. Anterograde labeling of terminals was done with 2% WGA-HRP injections in the cervical enlargement; thalamocortical cells were retrogradely labeled with fluorescent tracers. In one experiment, the contralateral;primary somatosensory cortex hand region (hSI) was injected to provide a direct comparison with hSII thalamic label. Both labeled cells and terminal-like structures were visualized in single thalamic sections and their numbers and positions quantitatively analyzed. The number of labeled cells within 100 microns from the STT terminals were counted as overlapping cells. Four thalamic nuclei, ventroposterior inferior (VPI), ventroposterior lateral (VPI), posterior nucleus (PO) and centrolateral nucleus (CL) combined to contain 86.5% of all hSII-projecting overlapping cells. Of all hSII-projecting thalamic overlapping cells, VPI contained the largest number (36.4% of the total)followed by the anterior portion of the posterior nuclear complex (POa; 20.4%), VPL (18.3%) and CL (11.4%). Results of the hSI injection show a different pattern of overlap in agreement with our earlier study. The relative distribution of overlapping cells was dependent on the antero-posterior position of the SII injections. The most anterior injections resulted in small numbers of labeled cells, with the majority of overlapping cells located in PO and CL. The more posterior injections resulted in overlapping cells mainly in VPI and VPL. The results indicate that, in the squirrel monkey, VPI, VPL, POa and CL relay nociceptive information from the spinal cord to the second somatosensory cortex.