Species-specific effects of mycorrhizal symbiosis on Populus trichocarpa after a lethal dose of copper
Species-specific effects of mycorrhizal symbiosis on Populus trichocarpa after a lethal dose of copper
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DOI：
10.1016/j.ecoenv.2024.116112
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发表时间：
2024-02-13
影响因子：
6.8
通讯作者：
Tibbett，Mark
中科院分区：
文献类型：
--
作者：
Soltangheisi，Amin;Hales-Henao，Aysha;Tibbett，Mark
杨树已被鉴定为重金属超积累植物，可用于植物修复。我们已经证实，它们与丛枝菌根真菌(AMF)的共生可能改变它们对土壤中过量重金属的吸收、耐受性和分布。在这项研究中，我们假设菌根共生提高了杨树对致死铜浓度的耐受性，但这种影响可能在不同的AMF物种之间有所不同。本试验采用完全随机设计，设对照(0 mg kg−1)、临界致死(72 9 mg kg−1)和超致死(6561 mg kg−1)三个铜处理水平和3个菌根处理(非菌根处理、不规则根际处理和漆酶拟青霉处理)，在生长箱中进行试验。施铜729 mg/kg−-1底物后，杨树不再生长，菌根共生不利于植物对铜的耐受。这可以用菌根真菌所遭受的毒性来解释。根际铜向地上部的转运增加了植物对根际铜的侵染。不规则性和P。紫胶漆分别在铜的阈值致死和超致死作用下。这一结果表明，菌根对杨树中铜分配的调节作用取决于真菌种类和底物铜浓度。在每个菌根处理内的多模型推理分析表明，在被R.不规则，较高水平的菌根定植可能会阻止铜向地上部的转移。我们在INP中没有观察到这种影响。漆属植物可能是由于相对较低的定殖率(14%)。根和地上部的养分浓度受施铜水平的影响，但不受菌根的影响。镁(Mg)、钾(K)和锰(Mn)的离子半径与铜相似，且具有共同的迁移机制，因此随着基质铜施用量的增加，根中镁、钾、锰的浓度也随之降低。基质铜水平与镁、钾、钙、铁、锌之间存在协同效应。根部铜浓度与根部K、Mn浓度呈负相关，地上部铜浓度与地上部Fe、K浓度呈正相关。总体而言，菌根共生具有提高植物健康及其在污染事件中对铜毒害的适应能力的潜力。然而，重要的是要注意到，这种共生关系的有效性在不同的菌根物种之间是不同的，并受到污染程度的影响。
Poplars have been identified as heavy metals hyperaccumulators and can be used for phytoremediation. We have previously established that their symbiosis with arbuscular mycorrhizal fungi (AMF) may alter their uptake, tolerance and distribution to excess concentrations of heavy metals in soils. In this study we hypothesised that mycorrhizal symbiosis improves the tolerance of poplars to lethal copper (Cu) concentrations, but this influence may vary among different AMF species. We conducted an experiment in a growth chamber with three Cu application levels of control (0 mg kg−1), threshold-lethal (729 mg kg−1) and supra-lethal (6561 mg kg−1), and three mycorrhizal treatments (non-mycorrhizal,Rhizophagus irregularis, andParaglomus laccatum) in a completely randomized design with six replications. The poplars did not grow after application of 729 mg Cu kg−1substrate, and mycorrhizal symbiosis did not help plants to tolerate this level of Cu. This can be explained by the toxicity suffered by mycorrhizal fungi. Translocation of Cu from roots to shoots increased when plants were colonised withR. irregularisandP. laccatumunder threshold-lethal and supra-lethal applications of Cu, respectively. This result shows that mycorrhizal mediation of Cu partitioning in poplars depends on the fungal species and substrate Cu concentration. Multi-model inference analysis within each mycorrhizal treatment showed that in plants colonised withR. irregularis, a higher level of mycorrhizal colonisation may prevent Cu transfer to the shoots. We did not observe this effect inP. laccatumplants probably due to the relatively low colonisation rate (14%). Nutrient concentrations in roots and shoots were impacted by applied substrate Cu levels, but not by mycorrhizas. Magnesium (Mg), potassium (K), and manganese (Mn) concentrations in roots reduced with enhancing applied substrate Cu due to their similar ionic radii with Cu and having common transport mechanism. Synergistic effect on shoot concentration between applied substrate Cu levels and Mg, K, calcium, iron (Fe), and zinc was observed. Root Cu concentration was inversely related with root K and Mn concentrations, and shoot Cu concentration had a positive correlation with shoot Fe and K concentrations. Overall, mycorrhizal symbiosis has the potential to enhance plant health and their resilience to Cu toxicity in contamination events. However, it is important to note that the effectiveness of this symbiotic relationship varies among different mycorrhizal species and is influenced by the level of contamination.