DNA vaccines: improving expression of antigens.
DNA vaccines: improving expression of antigens.
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DOI：
10.1186/1479-0556-1-2
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发表时间：
2003-09-16
期刊：
影响因子：
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通讯作者：
Titball, Richard W
中科院分区：
文献类型：
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作者：
Garmory, Helen S;Brown, Katherine A;Titball, Richard W
DNA疫苗接种是疫苗方法学中相对较新的发展。现在有可能对DNA疫苗的设计采取一种合理的逐步的方法。例如，策略可以包括在质粒骨架中掺入免疫刺激序列、共表达刺激分子、利用定位/分泌信号和利用适当的递送系统。然而，另一个重要的考虑因素是利用设计用于优化转基因表达的方法。在这篇综述中，我们讨论了调控元件，kozak序列和密码子优化在转基因表达的重要性。
DNA vaccination is a relatively recent development in vaccine methodology. It is now possible to undertake a rational step-by-step approach to DNA vaccine design. Strategies may include the incorporation of immunostimulatory sequences in the backbone of the plasmid, co-expression of stimulatory molecules, utilisation of localisation/secretory signals, and utilisation of the appropriate delivery system, for example. However, another important consideration is the utilisation of methods designed to optimise transgene expression. In this review we discuss the importance of regulatory elements, kozak sequences and codon optimisation in transgene expression.