PROPOSALS TO UNIFY THE GENERA BARTONELLA AND ROCHALIMAEA, WITH DESCRIPTIONS OF BARTONELLA-QUINTANA COMB-NOV, BARTONELLA-VINSONII COMB-NOV, BARTONELLA-HENSELAE COMB-NOV, AND BARTONELLA-ELIZABETHAE COMB-NOV, AND TO REMOVE THE FAMILY BARTONELLACEAE FROM THE ORDER RICKETTSIALES
PROPOSALS TO UNIFY THE GENERA BARTONELLA AND ROCHALIMAEA, WITH DESCRIPTIONS OF BARTONELLA-QUINTANA COMB-NOV, BARTONELLA-VINSONII COMB-NOV, BARTONELLA-HENSELAE COMB-NOV, AND BARTONELLA-ELIZABETHAE COMB-NOV, AND TO REMOVE THE FAMILY BARTONELLACEAE FROM THE ORDER RICKETTSIALES
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DOI：
10.1099/00207713-43-4-777
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发表时间：
1993-10-01
期刊：
影响因子：
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通讯作者：
STEIGERWALT, AG
中科院分区：
文献类型：
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作者：
BRENNER, DJ;OCONNOR, SP;STEIGERWALT, AG
DNA杂交数据（羟基磷灰石法，50至70 ℃）表明，普氏立克次体，立克次体科模式属的模式种，与Rochalimaea种的关系比以前认为的要小得多。在最佳DNA重排条件下，普氏立克次体与Rochalimaea种和Bartonella bacilliformis的相关性水平为0 ~ 14%，相关序列的差异为25.5%或更大。当使用严格的重新关联标准时，相关性水平为0至2%。巴尔通体属和Rochalimaea属目前被分类在立克次体目的不同科（巴尔通体科和立克次体科）中。根据DNA相关性数据，以前的16 S rRNA序列数据，鸟嘌呤加胞嘧啶含量和表型特征，无论是巴尔通体bacilliformis或Rochalimaea物种是密切相关的其他生物体目前分类在立克次体目。事实上，这些微生物的近亲是流产布鲁氏菌。因此，建议将巴尔通体科从立克次体目中删除。先前的16 S rRNA序列数据和DNA杂交数据显示，Bartonella bacilliformis和四种Rochalimaea物种之间存在高度相关性，表明这些物种是单一属的成员。建议将Rochalimaea属与Bartonella科Bartonella属合并。巴尔通体（Bartonella）的名称被保留为属名，因为它在命名上优先于Rochalimaea。这意味着必须为Rochalimaea物种创造新的组合。因此，建议建立巴尔通体quintana comb. nov.，vinsonii comb. nov.巴尔通体，汉赛巴尔通体和伊丽莎白巴尔通体（Bartonella elizabethae comb. nov.）
DNA hybridization data (hydroxyapatite method, 50 to 70-degrees-C) indicate that Rickettsia prowazekii, the type species of the type genus of the family Rickettsiaceae, is substantially less closely related to Rochalimaea species than was previously thought. The levels of relatedness of Rickettsia prowazekii to Rochalimaea species and to Bartonella bacilliformis under optimal conditions for DNA reassociation were 0 to 14%, with 25.5% or greater divergence in related sequences. When stringent reassociation criteria were used, the levels of relatedness were 0 to 2%. The genera Bartonella and Rochalimaea are currently classified in different families (the Bartonellaceae and the Rickettsiaceae) in the order Rickeusiales. On the basis of DNA relatedness data, previous 16S rRNA sequence data, guanine-plus-cytosine contents, and phenotypic characteristics, neither Bartonella bacilliformis nor Rochalimaea species are closely related to other organisms currently classified in the order Rickettsiales. In fact, the closest relative of these organisms is Brucella abortus. It is therefore proposed that the family Bartonellaceae should be removed from the order Rickettsiales. Previous 16S rRNA sequence data and DNA hybridization data revealed high levels of relatedness between Bartonella bacilliformis and the four Rochalimaea species, indicating that these species are members of a single genus. It is proposed that the genus Rochalimaea should be united with the genus Bartonella in the family Bartonellaceae. The name Bartonella is retained as the genus name since it has nomenclatural priority over the name Rochalimaea. This means that new combinations for the Rochalimaea species must be created. Proposals are therefore made for the creation of Bartonella quintana comb. nov., Bartonella vinsonii comb. nov., Bartonella henselae comb. nov., and Bartonella elizabethae comb. nov.