Genetic mapping and expressivity of a wheat multi-pistil gene in mutant 12TP
Genetic mapping and expressivity of a wheat multi-pistil gene in mutant 12TP
复制标题
小麦多雌蕊突变体12TP基因的遗传图谱和表达量
DOI：
10.1016/s2095-3119(18)61935-5
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发表时间：
2019-03-01
影响因子：
4.8
通讯作者：
Niu Ji-shan
中科院分区：
文献类型：
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作者：
Zhu Xin-xin;Ni Yong-jing;Niu Ji-shan
我们鉴定出一种小麦(Triticum aestivum L.)2012年从F-2育种群体中发现了一个多雌蕊突变体，命名为12TP(一花三雌蕊)。遗传分析表明，多雌蕊性状由1个显性基因控制。利用来自原始突变体12TP的纯合正常和多雌株系(近等基因系；NILs)，利用SSR标记定位了染色体臂2d1上的12TP基因座。4个SSR标记与12TP连锁，其顺序为Xcfd233->；Xcfd62-12TP->；Xwmc41->；Xcfd168，分别位于15.85、10.47、2.89、10.37 cM。在7个纯合子12TP品系中，‘一花三雌蕊’的平均遗传表现率在98%以上，而在杂合的F-1植株中，平均遗传表现率约为49%。因此，12TP是一个不同于已报道的所有多雌蕊突变体的半显性基因座。突变体12TP是研究小麦花发育和选育高产小麦的有用新种质。
We identified a wheat (Triticum aestivum L.) multi-pistil mutant from an F-2 breeding population in 2012, named 12TP (three pistils in one floret). Genetic analysis showed that one dominant gene locus controlled the multi-pistil trait. Using homozygous normal and multi-pistil lines (near-isogenic lines; NILs) derived from the original mutant 12TP, a simple sequence repeat (SSR) marker assay located the 12TP locus on chromosome arm 2DL. Four SSR markers were linked to 12TP and their order was Xcfd233 -> Xcfd62-12TP -> Xwmc41 -> Xcfd168 at 15.85, 10.47, 2.89, and 10.37 cM, respectively. The average genetic expressivity of the trait 'three pistils in one floret' was more than 98% in seven homozygous 12TP lines; however, the average genetic expressivity in heterozygous F-1 plants was about 49%. Thus, the 12TP is a semi-dominant gene locus, which differ from all previously reported multi-pistil mutants. Mutant 12TP is a new useful germplasm for study of wheat floral development and for breeding of high yield wheat.