3 EPITOPE-SPECIFIC MONOCLONAL-ANTIBODIES AGAINST THE HAPTEN PENICILLIN
3 EPITOPE-SPECIFIC MONOCLONAL-ANTIBODIES AGAINST THE HAPTEN PENICILLIN
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DOI：
10.1159/000233659
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发表时间：
1985-01-01
期刊：
影响因子：
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通讯作者：
BOORSMA, DM
中科院分区：
文献类型：
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作者：
DEHAAN, P;DEJONGE, AJR;BOORSMA, DM
制备了3株抗青霉酰基的单抗(Pen 4、Pen 7和Pen 9)，用于研究该基团的抗原性。在ELISA法中，Pen 4与载体结合的青霉素衍生物的结合依赖于衍生物中侧链的结构。因此，Pen 4识别这个侧链。根据竞争酶免疫分析中青霉素衍生物与载体结合的差异，得出结论：Pen 7主要识别青霉素衍生物与载体结合产生的新的抗原决定簇。Pen 9与载体结合的青霉素衍生物的结合不受侧链性质的影响。影响的派生对约束的性质也不是约束的或自由的。因此，Pen 9主要识别青霉素的噻唑环。因此，这项研究表明，在苄基青霉烷基中，至少可以识别三个表位。
Three monoclonal antibodies (Pen 4, Pen 7 and Pen 9) were raised against the benzylpenicilloyl group and used to investigate the antigenicity of this group. The binding of Pen 4 to carrier-bound penicillin derivatives was shown in an ELISA to be dependent on the structure of the side chain in the derivative. Hence Pen 4 recognizes this side chain. From the difference in binding to carrier-bound penicillin derivatives in a competitive enzyme immunoassay it was concluded that Pen 7 mainly recognizes the new antigenic determinant which emerges from the binding of the penicillin derivative to a carrier. The binding of Pen 9 to carrier-bound penicillin derivatives was not influenced by the nature of the side chain. Neither was the bound or free nature of the derivative of influence on the binding. Therefore it is concluded that Pen 9 mainly recognizes the thiazolidin ring of penicillin. This study thus shows that in the benzylpenicilloyl group at least three epitopes can be recognized.