Effect of allopurinol on neutrophil superoxide production, chemotaxis, or degranulation.
Effect of allopurinol on neutrophil superoxide production, chemotaxis, or degranulation.
复制标题
别嘌呤醇对中性粒细胞超氧化物产生、趋化性或脱颗粒的影响。
DOI：
10.1016/0006-2952(85)90229-1
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发表时间：
1985
影响因子：
5.8
通讯作者：
J. McCord
中科院分区：
文献类型：
--
作者：
H. P. Jones;M. Grisham;S. Bose;V. A. Shannon;A. Schott;J. McCord
最近检测别嘌醇对白细胞杀菌作用的研究[Tubaroet al.，Biochem.Pharmac.29,3018(1980)；Tritsch和Neiswander，Life Science.32,1359(1983)]被那些作者解释为证明黄嘌呤氧化酶是激活的白细胞中主要的产生超氧化物的酶。为了验证黄嘌呤氧化酶参与人中性粒细胞产生超氧化物的说法，测定了中性粒细胞黄嘌呤氧化酶的含量，并研究了别嘌醇对中性粒细胞功能的影响，包括超氧化物的产生。中性粒细胞被发现含有不足以解释与中性粒细胞激活相关的超氧化物流通量的黄嘌呤氧化酶水平。别嘌醇不能抑制酵母多糖、佛波酯或甲硫基亮氨酸苯丙氨酸诱导的超氧化物生成。此外，别嘌醇既不影响趋化性，也不影响脱颗粒。别嘌醇对超氧化物介导的角叉菜胶致大鼠足肿胀的阻断作用也不明显。这些研究被解释为黄嘌呤氧化酶不是激活的中性粒细胞中主要的超氧化物生成系统的证据，正如其他人所说的那样。
Recent studies examining the effect of allopurinol on bacteral killing by leukocytes [Tubaroet al., Biochem. Pharmac.29, 3018 (1980); Tritsch and Neiswander,Life Sci.32, 1359 (1983)] have been interpreted by those authors as proof that xanthine oxidase is the major Superoxide producing enzyme in activated leukocytes. To test the assertion that xanthine oxidase is involved in the production of Superoxide by activated human neutrophils, the xanthine oxidase content of neutrophils was measured, and the effect of allopurinol on neutrophil functions, including Superoxide production, was studied. Neutrophils were found to contain a level of xanthine oxidase insufficient to account for the flux of Superoxide associated with neutrophil activation. Allopurinol did not inhibit Superoxide production induced by opsonized zymosan, phorbol myristic acetate, or formylmethionylleucylphenylalanine. Furthermore, neither chemotaxis nor degranulation was affected by allopurinol. Allopurinol was also found ineffective in blocking superoxide-mediated carrageenan-induced foot edema in the rat. These studies are interpreted as evidence that xanthine oxidase is not a major superoxide-generating system in activated neutrophils as has been suggested by others.