miR-1231 Is Downregulated in Prostate Cancer with Prognostic and Functional Implications
miR-1231 Is Downregulated in Prostate Cancer with Prognostic and Functional Implications
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DOI：
10.1159/000504606
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发表时间：
2020-03-01
影响因子：
2.4
通讯作者：
Zhao, Dan
中科院分区：
文献类型：
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作者：
Wang, Yitao;Zhang, Qingling;Zhao, Dan
背景：我们的研究调查了前列腺癌组织和细胞系中 miR-1231 的表达水平，并探讨了其潜在的预后意义及其对前列腺癌细胞的功能影响。方法：采用 qRT-PCR 检测前列腺癌组织和前列腺癌细胞系中 miR-1231 的表达水平。使用 Kaplan-Meier 曲线和 Cox 回归分析评估 miR-1231 在前列腺癌中的预后意义。评估 miR-1231 异位表达或抑制对前列腺癌细胞系侵袭性肿瘤行为（细胞增殖、迁移和侵袭）的影响涉及 CCK-8 和 Transwell 测定。结果：miR-1231 表达在前列腺癌组织和细胞系中均下调。 miR-1231的下调与淋巴结转移、较高的TNM分期、较高的临床分期和较短的总生存期显着相关。 miR-1231 的表达被预测为前列腺癌患者的预后因素。此外，我们发现 miR-1231 的过度表达可抑制体外前列腺癌细胞系的增殖、迁移和侵袭。 EGFR 是 miR-1231 的直接靶点。结论：总而言之，我们的结果表明 miR-1231 表达在前列腺癌中发挥肿瘤抑制作用。它在前列腺癌中下调，可能通过靶向EGFR来抑制前列腺癌细胞的增殖、迁移和侵袭，并且可能成为未来前列腺癌治疗的预后生物标志物和潜在的治疗策略。
Background: Our study investigated the expression levels of miR-1231 in prostate cancer tissues and cell lines and explored its potential prognostic significance as well as its functional effects on prostate cancer cells. Methods: miR-1231 expression levels were detected in prostate cancer tissues and prostate cancer cell lines using qRT-PCR. The prognostic significance of miR-1231 in prostate cancer was assessed using the Kaplan-Meier curve and Cox regression analyses. Evaluation of the impact of ectopic expression or inhibition of miR-1231 on aggressive tumor behavior (cellular proliferation, migration, and invasion) of prostate cancer cell lines involved the CCK-8 and Transwell assays. Results: miR-1231 expression was downregulated in both prostate cancer tissues and cell lines. Downregulation of miR-1231 was significantly associated with lymph node metastasis, higher TNM stage, higher clinical stage, and shorter overall survival. The expression of miR-1231 was predicted as a prognostic factor for prostate cancer patients. Additionally, we found overexpression of miR-1231 suppressed proliferation, migration, and invasion of prostate cancer cell linesin vitro. EGFR was a direct target of miR-1231. Conclusion: Taken together, our results indicate that miR-1231 expression plays a tumor-suppressive role in prostate cancer. It is downregulated in prostate cancer and may suppress prostate cancer cell proliferation, migration, and invasion by targeting EGFR, and it may be a prognostic biomarker and potential therapeutic strategy for prostate cancer treatment in the future.