Preparation and HPLC evaluation of a new 1,3-alternate 25,27-bis-[p-chlorobenzyloxy]-26,28-bis-[3-propyloxy]-calix[4]arene silica bonded stationary phase.
Preparation and HPLC evaluation of a new 1,3-alternate 25,27-bis-[p-chlorobenzyloxy]-26,28-bis-[3-propyloxy]-calix[4]arene silica bonded stationary phase.
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DOI：
10.1002/jssc.200700504
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发表时间：
2008-04
影响因子：
3.1
通讯作者：
M. Śliwka-Kaszyńska;Sebastian Karaszewski
中科院分区：
文献类型：
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作者：
M. Śliwka-Kaszyńska;Sebastian Karaszewski
合成了 1,3-交替 25,27-双-[对氯苄氧基]-26,28-双-[3-丙氧基]-杯[4]芳烃键合硅胶固定相，进行了结构表征，并将其用作高效液相色谱中的选择器。该相的选择性研究使用芳香族位置异构体、烷基苯、多核芳香烃、磺酰胺和非甾体抗炎药作为分析物。研究了有机改性剂含量和流动相 pH 对所选芳香族位置异构体保留和选择性的影响。对新型相与 1,3-替代 25,27-二-[苄氧基]-26,28-双-[3-丙氧基]-杯[4]芳烃相和市售 RP-苯基相进行了选择性比较。还讨论了保留机制。结果表明，杯芳烃固定相的行为类似于反相填料；然而，分离过程似乎还涉及其他保留机制。
The 1,3-alternate 25,27-bis-[p-chlorobenzyloxy]-26,28-bis-[3-propyloxy]-calix[4]arene-bonded silica gel stationary phase was synthesized, structurally characterized, and used as a selector in high performance liquid chromatography. Selectivity studies on that phase used aromatic positional isomers, alkylbenzenes, polynuclear aromatic hydrocarbons, sulfonamides, and non-steroidal anti-inflammatory drugs as analytes. The effects of organic modifier content and pH of the mobile phase on retention and selectivity of selected aromatic positional isomers were studied. Selectivity comparisons of the novel phase vs. 1,3-alternate 25,27-di-[benzyloxy]-26,28-bis-[3-propyloxy]-calix[4]arene phase and commercially available RP-Phenyl phases were performed. The retention mechanism was also discussed. The results indicated that the calixarene stationary phase behaves like a reversed-phase packing; however, other retention mechanisms seem to be involved in the separation process.