Activation of c-Src is inversely correlated with biological aggressiveness of breast carcinoma
Activation of c-Src is inversely correlated with biological aggressiveness of breast carcinoma
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DOI：
10.1023/a:1020860221099
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发表时间：
2002-12-01
影响因子：
3.8
通讯作者：
Matsuura, N
中科院分区：
文献类型：
--
作者：
Ito, Y;Kawakatsu, H;Matsuura, N
为了研究c-Src是否参与乳腺癌的发生和发展，我们检测了手术切除的人类乳房标本组织切片中活化c-Src的表达。首先，我们利用western blotting技术从人乳腺癌中建立的6株细胞系，证实了抗体对活化的c-Src（克隆28）的特异性。正如预期的那样，在所有测试的细胞系中都检测到活化的c-Src为60 kDa带。免疫荧光分析表明，活化的c-Src主要存在于这些细胞的细胞质中。然后，我们设计了73人乳腺癌组织的免疫组织化学研究。癌巢附近正常乳腺的腺上皮细胞和肌上皮细胞及浸润间质细胞中活化的c-Src呈阴性。相比之下，73例乳腺癌中有37例（50.7%）活化的c-Src阳性，这种阳性染色与Ki-67标记指数呈负相关(p
In order to investigate whether c-Src is involved in carcinogenesis and progression of breast carcinoma, we examined the expression of activated c-Src in tissue sections from surgically resected human breast specimens. First, we confirmed the specificity of the antibody against activated c-Src (Clone 28) using six cell lines established from human breast carcinomas by western blotting. As expected, activated c-Src was detected as a 60 kDa band in all cell lines tested. Immunofluorescence analysis demonstrated that the activated c-Src was mainly observed in cytoplasms of these cells. Then, we designed an immunohistochemical study with 73 human breast carcinoma tissues. Glandular epithelial and myoepithelial cells in normal mammary glands adjacent to carcinoma nests and infiltrating stromal cells were negative for activated c-Src. In contrast, 37 of the 73 breast carcinoma tested (50.7%) were positive for activated c-Src, and this positive staining was inversely correlated with Ki-67 labeling index (p