Alteration of complement hemolytic activity in different trauma and sepsis models
Alteration of complement hemolytic activity in different trauma and sepsis models
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DOI：
10.2147/jir.s31787
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发表时间：
2012-01-01
影响因子：
4.5
通讯作者：
Bahrami, Soheyl
中科院分区：
文献类型：
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作者：
Ehrnthaller, Christian;Amara, Umme;Bahrami, Soheyl
补体激活参与先天免疫起关键作用的各种疾病。然而，其病理生理学相关性尚不清楚。实验模型已被广泛用于表征补体激活在不同病理条件下的作用，如低氧血症、缺血和再灌注、组织损伤和多种微生物侵袭。然而，补体状态和功能的筛选强烈依赖于用于采样和测量补体的实验室特定技术，使得难以比较不同实验室中发现的结果。因此，我们通过测量补体溶血活性（CH 50）在各种动物模型中的隔离缺血再灌注（I/R：肾，肝，肠），出血性创伤性休克（HTS），内毒素休克（LPS），脓毒症（CLP）的补体功能进行了评估。补体激活在孤立的缺血和再灌注模型中不太明显，而在HTS、CLP和LPS后早期观察到强烈的补体反应。综上所述，CH 50是一种成熟、快速且具有成本效益的补体功能筛选方法。然而，由于我们在临床相关的动物模型中获得了不同的结果，因此有必要使用特异性补体因子分析进行进一步区分。
Complement activation is involved in various diseases in which innate immunity plays a crucial role. However, its pathophysiological relevance is not clearly understood. Experimental models have been widely used to characterize the role of complement activation under different pathological conditions, such as hypoxemia, ischemia and reperfusion, tissue damage, and polymicrobial invasion. Screening of the complement status and function is, however, strongly dependent on the laboratory-specific techniques being used to sample and measure complement, making it difficult to compare the results found in different laboratories. Therefore, we evaluated complement function by measuring complement hemolytic activity (CH50) in various animal models of isolated ischemia reperfusion (I/R: kidney, liver, gut), hemorrhagic traumatic shock (HTS), endotoxic shock (LPS), and sepsis (CLP). Complement activation was less pronounced in isolated models of ischemia and reperfusion, whereas a strong complement response was observed early after HTS, CLP, and LPS. In summary, CH50 is a well-established, quick, and cost-effective screening method of complement function. However, because we obtained different results in clinically relevant animal models, further differentiation using specific complement factor analysis is necessary.