Multiparameter flow cytometry for the identification of the Waldenstrom's clone in IgM-MGUS and Waldenstrom's Macroglobulinemia: new criteria for differential diagnosis and risk stratification
Multiparameter flow cytometry for the identification of the Waldenstrom's clone in IgM-MGUS and Waldenstrom's Macroglobulinemia: new criteria for differential diagnosis and risk stratification
复制标题
DOI：
10.1038/leu.2013.124
复制
发表时间：
2014-01-01
期刊：
影响因子：
11.4
通讯作者：
San Miguel, J. F.
中科院分区：
文献类型：
--
作者：
Paiva, B.;Montes, M. C.;San Miguel, J. F.
虽然多参数流式细胞术（MFC）已证明临床意义不明的单克隆丙种球蛋白病（MGUS）/骨髓瘤的相关性，免疫表型研究的全谱瓦尔登斯特伦巨球蛋白血症（WM）仍然很少。本文对244例新诊断的免疫球蛋白M（IgM）单克隆蛋白患者的骨髓样本进行了全面的MFC分析，其中包括67例IgM-MGUS，77例阴燃和100例症状性WM。我们的结果显示从IgM-MGUS到郁积和症状性WM，B细胞的数量和轻链同种型阳性的B细胞逐渐增加（P <0.05），10%的B细胞或100%的轻链同种型阳性的B细胞（P <0.05）。
Although multiparameter flow cytometry (MFC) has demonstrated clinical relevance in monoclonal gammopathy of undetermined significance (MGUS)/myeloma, immunophenotypic studies on the full spectrum of Waldenstrom's Macroglobulinemia (WM) remain scanty. Herein, a comprehensive MFC analysis on bone marrow samples from 244 newly diagnosed patients with an immunoglobulin M (IgM) monoclonal protein was performed, including 67 IgM-MGUS, 77 smoldering and 100 symptomatic WM. Our results show a progressive increase on the number and light-chain-isotype-positive B-cells from IgM-MGUS to smoldering and symptomatic WM (P 10% B cells or 100% light-chain-isotype-positive B-cells (P