MHC polymorphism and disease resistance to Vibrio anguillarum in 12 selective Japanese flounder (Paralichthys olivaceus) families
MHC polymorphism and disease resistance to Vibrio anguillarum in 12 selective Japanese flounder (Paralichthys olivaceus) families
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12个牙鲆科MHC多态性及对鳗弧菌的抗病性
DOI：
10.1016/j.fsi.2008.05.007
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发表时间：
2008-09-01
影响因子：
4.7
通讯作者：
Tian, Yong-sheng
中科院分区：
文献类型：
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作者：
Xu, Tian-jun;Chen, Song-lin;Tian, Yong-sheng
在主要组织相容性复合体（MHC）类IIB的遗传变异进行了测试，在日本比目鱼（Paralichthys olivaceus）的生存挑战后，细菌感染。材料包括6000日本比目鱼从60个家庭的挑战与鳗弧菌，这会导致显着不同的死亡率比目鱼家庭。使用序列分析筛选来自6个高抗性（HR）和6个低抗性（LR）家族的5个个体的MHC IIB类基因型。结果表明，在牙鲆群体中，MHC-IIB基因和至少3个位点存在高度多态性，d（N）位点的出现频率显著高于d（S）位点。在60个个体中，共发现76个等位基因，其中15个等位基因用于研究等位基因与抗病性的关联。我们发现日本比目鱼对鳗弧菌引起的传染病的抗性与MHC Ⅱ B类基因多态性之间存在高度显著的相关性。有些等位基因在HR和LR家系中同时出现，有些等位基因仅在HR或LR家系中发现。其中一个等位基因Paol-DAB*4301在HR家族中的流行率显著高于LR家族（P = 0.023）。Paol-DAB*0601、Paol-DAB*0801、Paol-DAB*2001、Paol-DAB*3803在两个HR家系中出现频率较高。在3个LR家系中发现1个等位基因Paol-DAB*1601。MHC Ⅱ B类等位基因遗传稳定，具有Paol-DAB*4301等位基因的家系对鳗弧菌的抗性较高。本研究证实了MHC Ⅱ B类基因的等位基因与抗病性的相关性，同时也检测到了一些可能与细菌感染高抗性相关的等位基因。这些抗病相关的MHC标记可用于比目鱼的分子标记辅助选择育种。(c)2008爱思唯尔有限公司版权所有。
Genetic variation in the major histocompatibility complex (MHC) class IIB was tested in Japanese flounder (Paralichthys olivaceus) for survival after challenge with bacterial infection. The material consisted of 6000 Japanese flounder from 60 families challenged with Vibrio anguillarum, which causes significantly different mortality in flounder families. Five individuals from each of six high-resistance (HR) and six low-resistance (LR) families were screened for their MHC class IIB genotypes using sequence analysis. High polymorphism of MHC IIB gene and at least three loci were discovered in Japanese flounder and the rate of d(N) occurred at a significantly higher frequency than that of d(S) in PBR. Among 60 individuals, 76 alleles were discovered and 15 alleles were used to study associations between alleles and resistance to disease. We found highly significant associations between resistance towards infectious disease caused by V. anguillarum and MHC class IIB polymorphism in Japanese flounder. Some alleles appeared in both HR and LR families, while some alleles were only discovered in HR or LR families. One allele, Paol-DAB*4301, was significantly more prevalent in HR families than in LR families (P = 0.023). Paol-DAB*0601, Paol-DAB*0801, Paol-DAB*2001, Paol-DAB*3803 were discovered in two HR families with high frequency. One allele, Paol-DAB*1601, was discovered in three LR families. The steady heredity of MHC class IIB alleles was observed, and the family having Paol-DAB*4301 alleles was confirmed with higher resistance to V. anguillarum. This study confirmed the association between alleles of MHC class IIB gene and disease resistance, and also detected some alleles which might be correlated with high bacterial infection resistance. The disease resistance-related MHC markers could be used for molecular marker-assisted selective breeding in the flounder. (c) 2008 Elsevier Ltd. All rights reserved.