A portable electrochemical sensing platform for serotonin detection based on surface-modified carbon fiber microelectrodes
A portable electrochemical sensing platform for serotonin detection based on surface-modified carbon fiber microelectrodes
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DOI：
10.1039/d2ay01627c
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发表时间：
2023-01-26
影响因子：
3.1
通讯作者：
Ghodssi，Reza
中科院分区：
文献类型：
--
作者：
Han，Jinjing;Stine，Justin M.;Ghodssi，Reza
5-羟色胺(5-HT)是人体内重要的神经递质之一，调节多种生理功能。5-羟色胺动态平衡的破坏可能导致严重的健康问题，包括神经退行性疾病、抑郁症和5-羟色胺综合征。检测尿液等生物体液中的5-羟色胺浓度是早期诊断这些疾病的潜在解决方案。在这项研究中，我们开发了一种新颖、简单、低成本的电化学传感平台，该平台包括一个带有定制电极的便携式工作站，用于检测人工生物液中的5-羟色胺。Nafion/碳纳米管(CNTs)和电化学修饰的碳纤维微电极(Nafion-CNT/EC CFME)表现出更高的5-羟色胺灵敏度和选择性。结合定制的银/氯化银参比电极和铂对电极，便携式5-羟色胺传感平台的灵敏度为0.074μAμM−1，检测限为140nM。该系统还被评估为测量人工尿样中添加的5-羟色胺，显示出几乎完全的回收率。这些令人满意的结果表明，便携式系统具有优异的性能，证实了5-羟色胺检测的可行性，可以用于实际生物样品的护理点分析。
Serotonin (5-HT) is one of the key neurotransmitters in the human body, regulating numerous physiological functions. A disruption in 5-HT homeostasis could result in serious health problems, including neurodegenerative disorders, depression, and 5-HT syndrome. Detection of 5-HT concentrations in biological fluids, such as urine, is a potential solution for early diagnosis of these diseases. In this study, we developed a novel, simple, and low-cost electrochemical sensing platform consisting of a portable workstation with customized electrodes for 5-HT detection in artificial biological fluids. Nafion/carbon nanotubes (CNTs) and electrochemically modified carbon fiber microelectrodes (Nafion–CNT/EC CFMEs) displayed improved 5-HT sensitivity and selectivity. Together with a customized Ag/AgCl reference electrode and Pt counter electrode, the portable 5-HT sensing platform had a sensitivity of 0.074 μA μM−1 and a limit of detection (LOD) of 140 nM. This system was also assessed to measure 5-HT spiked in artificial urine samples, showing nearly full recovery rates. These satisfactory results demonstrated that the portable system exhibits outstanding performance and confirmed the feasibility of 5-HT detection, which can be used to provide point-of-care analysis in actual biological samples.