Carbamoylcholine and gastrin induce inositol lipid turnover in canine gastric parietal cells.
Carbamoylcholine and gastrin induce inositol lipid turnover in canine gastric parietal cells.
复制标题
氨基甲酰胆碱和胃泌素诱导犬胃壁细胞中的肌醇脂质周转。
DOI：
10.1152/ajpgi.1988.255.1.g99
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Yamada,T
中科院分区：
文献类型：
--
作者：
Chiba,T;Fisher,SK;Park,J;Seguin,EB;Agranoff,BW;Yamada,T
在富含离体犬胃壁细胞的制剂中，研究了肌醇磷脂周转在介导酸分泌中的潜在作用。氨甲酰胆碱（卡巴胆碱）和胃泌素对壁细胞摄取[14 C]氨基比林的刺激作用与细胞磷脂酰肌醇含量的剂量和时间依赖性选择性减少有关，尽管磷酸肌醇的特定脂肪酸组成没有改变。[3 H]肌醇磷酸积累在细胞预标记的[3 H]肌醇的分析表明，增加标记的肌醇三磷酸与卡巴胆碱或胃泌素孵育5分钟。此外，壁细胞在培养基中含有[32 P]正磷酸盐预孵育后，两种促分泌素引起的32 P标记的磷脂酰肌醇4，5-二磷酸和伴随的磷脂酸标记的增加时间依赖性的减少。这些数据表明，卡巴胆碱和胃泌素的酸性促分泌作用与主要由壁细胞组成的制剂中细胞肌醇磷脂的周转相关。
The potential role of inositol phospholipid turnover in mediating acid secretion was examined in a preparation enriched for isolated canine gastric parietal cells. The stimulatory effects of carbamoylcholine (carbachol) and gastrin on parietal cell uptake of [14C]aminopyrine were linked to dose- and time-dependent selective reduction in cellular phosphatidylinositol content, although the specific fatty acid composition of the phosphoinositides was not altered. Analysis of [3H]inositol phosphates accumulated in cells prelabeled with [3H]inositol revealed an increase in labeled inositol trisphosphate by 5 min of incubation with either carbachol or gastrin. Furthermore, after preincubation of parietal cells in medium containing [32P]orthophosphate, the two secretagogues elicited a time-dependent decrease in 32P labeling of phosphatidylinositol 4,5-bisphosphate and concomitant increase in labeling of phosphatidic acid. These data demonstrate that the acid secretagogue actions of carbachol and gastrin are correlated with turnover of cellular inositol phospholipids in a preparation consisting predominantly of parietal cells.