Alternative splicing regulation of membrane trafficking genes during myogenesis.
Alternative splicing regulation of membrane trafficking genes during myogenesis.
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DOI：
10.1261/rna.078993.121
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发表时间：
2022-04
期刊：
影响因子：
--
通讯作者：
Giudice J
中科院分区：
文献类型：
--
作者：
Hinkle ER;Wiedner HJ;Torres EV;Jackson M;Black AJ;Blue RE;Harris SE;Guzman BB;Gentile GM;Lee EY;Tsai YH;Parker J;Dominguez D;Giudice J
选择性剪接转换发生在器官发育期间，并且在许多疾病中，剪接程序恢复到胎儿同种型表达。我们以前发现，广泛的剪接变化发生在出生后的小鼠心脏发育的基因编码的蛋白质参与囊泡介导的贩运。然而，这种拼接贩运网络的调控机制尚不清楚。在这里，我们发现，膜运输基因是选择性剪接的组织特异性的方式，与横纹肌表现出最高水平的选择性外显子包含。用染色质修饰药物治疗分化的肌细胞改变了肌细胞中的外显子包含。对几种RNA结合蛋白的研究表明，聚嘧啶束结合蛋白1（PTBP1）和震颤调节肌生成过程中运输基因的剪接，并且去除PTBP1基序阻止PTBP1结合其RNA靶。这些发现增强了我们对膜运输蛋白的发育剪接调控的理解，这可能对肌肉疾病的发病机制有影响。
Alternative splicing transitions occur during organ development, and, in numerous diseases, splicing programs revert to fetal isoform expression. We previously found that extensive splicing changes occur during postnatal mouse heart development in genes encoding proteins involved in vesicle-mediated trafficking. However, the regulatory mechanisms of this splicing-trafficking network are unknown. Here, we found that membrane trafficking genes are alternatively spliced in a tissue-specific manner, with striated muscles exhibiting the highest levels of alternative exon inclusion. Treatment of differentiated muscle cells with chromatin-modifying drugs altered exon inclusion in muscle cells. Examination of several RNA-binding proteins revealed that the poly-pyrimidine tract binding protein 1 (PTBP1) and quaking regulate splicing of trafficking genes during myogenesis, and that removal of PTBP1 motifs prevented PTBP1 from binding its RNA target. These findings enhance our understanding of developmental splicing regulation of membrane trafficking proteins which might have implications for muscle disease pathogenesis.
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影响因子：
16.6
作者：
Freyermuth F;Rau F;Kokunai Y;Linke T;Sellier C;Nakamori M;Kino Y;Arandel L;Jollet A;Thibault C;Philipps M;Vicaire S;Jost B;Udd B;Day JW;Duboc D;Wahbi K;Matsumura T;Fujimura H;Mochizuki H;Deryckere F;Kimura T;Nukina N;Ishiura S;Lacroix V;Campan-Fournier A;Navratil V;Chautard E;Auboeuf D;Horie M;Imoto K;Lee KY;Swanson MS;de Munain AL;Inada S;Itoh H;Nakazawa K;Ashihara T;Wang E;Zimmer T;Furling D;Takahashi MP;Charlet-Berguerand N
通讯作者：
Charlet-Berguerand N
影响因子：
16
作者：
Dujardin, Gwendal;Lafaille, Celina;Kornblihtt, Alberto R.
通讯作者：
Kornblihtt, Alberto R.
DOI：
10.1016/j.biocel.2018.10.004
发表时间：
2018-12-01
影响因子：
4
作者：
Blue, R. Eric;Koushik, Amrita;Giudice, Jimena
通讯作者：
Giudice, Jimena
DOI：
10.1083/jcb.200108102
发表时间：
2001-12-10
期刊：
The Journal of cell biology
影响因子：
--
作者：
Barr FA;Preisinger C;Kopajtich R;Körner R
通讯作者：
Körner R
影响因子：
4.5
作者：
Boehm, Johann;Vasli, Nasim;Laporte, Jocelyn
通讯作者：
Laporte, Jocelyn