Mei4p coordinates the onset of meiosis I by regulating cdc25+ in fission yeast
Mei4p coordinates the onset of meiosis I by regulating cdc25+ in fission yeast
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DOI：
10.1073/pnas.0702906104
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发表时间：
2007-09-11
影响因子：
11.1
通讯作者：
Murakami, Hiroshi
中科院分区：
文献类型：
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作者：
Murakami-Tonami, Yuko;Yamada-Namikawa, Chisato;Murakami, Hiroshi
激酶 Cdc2p 是真核生物有丝分裂和减数分裂过程中进入核分裂并进行核分裂的中央调节因子。 Cdc2p 在有丝分裂开始时通过酪氨酸 15 去磷酸化而被激活，其磷酸化状态主要由激酶 Wee1p 和磷酸酶 Cdc25p 决定。在裂殖酵母中，叉头型转录因子 Mei4p 是减数分裂过程中许多基因表达所必需的，mei4 突变细胞在减数分裂 1 之前停滞。然而，mei4 细胞中细胞周期停滞的机制仍不清楚。我们现在表明，cdc25(+)是Mei4p控制进入减数分裂1的重要靶标。Cdc2p在酪氨酸15上的强制去磷酸化从而诱导mei4突变细胞中的减数分裂1没有延迟，尽管没有形成孢子。我们认为 Mei4p 通过与其他减数分裂事件协调激活 cdc25+ 转录来充当减数分裂 I 的限速调节因子。
The kinase Cdc2p is a central regulator of entry into and progression through nuclear division during mitosis and meiosis in eukaryotes. Cdc2p is activated at the onset of mitosis by dephosphorylation on tyrosine-15, the phosphorylation status of which is determined mainly by the kinase Wee1p and the phosphatase Cdc25p. In fission yeast, the forkhead-type transcription factor Mei4p is required for expression of many genes during meiosis, with mei4 mutant cells arresting before meiosis 1. The mechanism of cell cycle arrest in mei4 cells has remained unknown, however. We now show that cdc25(+) is an important target of Mei4p in control of entry into meiosis 1. Forced dephosphorylation of Cdc2p on tyrosine-15 thus induced meiosis 1 in mei4 mutant cells without a delay, although no spores were formed. We propose that Mei4p acts as a rate-limiting regulator of meiosis I by activating cdc25+ transcription in coordination with other meiotic events.