High-resolution mapping of GRH6, a gene from Oryza nivara (Sharma et Shastry) conferring resistance to green rice leafhopper (Nephotettix cincticeps Uhler)
High-resolution mapping of GRH6, a gene from Oryza nivara (Sharma et Shastry) conferring resistance to green rice leafhopper (Nephotettix cincticeps Uhler)
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DOI：
10.1270/jsbbs.19029
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发表时间：
2019-01-01
期刊：
影响因子：
2.4
通讯作者：
Yasui, Hideshi
中科院分区：
文献类型：
--
作者：
Cong Nguyen Phi;Fujita, Daisuke;Yasui, Hideshi
绿色稻叶蝉（Nephotettix cincticeps Uhler）是栽培稻的主要害虫，遍布东亚的温带地区野生稻中已经发现了GRH抗性，但野生稻GRH抗性的遗传基础还不清楚。在感病粳稻品种台中65（T65）与O. nivara登录号IRGC 105715。qGRH4.2在来自两个抗GRH的BC 3F 1植物的BC 3F 2群体中被鉴定为介于标记RM 5414和C60248之间的GRH 6。在高分辨率定位中，GRH 6区域被限定在标记G6-c60 k和7 L16 f之间，并且对应于'日本晴'基因组的31.2kbp区域。了解GRH抗性的遗传基础将有助于利用GRH抗性基因进行水稻分子标记辅助育种。
The green rice leafhopper (GRH), Nephotettix cincticeps Uhler, is a major insect pest of cultivated rice, Oryza sativa L., throughout the temperate regions of East Asia. GRH resistance had been reported in the wild species Oryza nivara but genetic basis of GRH resistance in wild rice accession has not been clarified. Here, we found a major QTL, qGRH4.2, on chromosome 4 conferred GRH resistance with 14.1 of the logarithm of odds (LOD) score explaining 67.6% of phenotypic variance in the BC1F1 population derived from a cross between the susceptible japonica cultivar 'Taichung 65' (T65) and O. nivara accession IRGC105715. qGRH4.2 has been identified as GRH6 between the markers RM5414 and C60248 in a BC3F2 population derived from two BC3F1 plants resistant to GRH. In a high-resolution mapping, the GRH6 region was delimited between the markers G6-c60k and 7L16f, and corresponded to an 31.2-kbp region of the 'Nipponbare' genome. Understanding the genetic basis of GRH resistance will facilitate the use of GRH resistance genes in marker-assisted breeding in rice.