Genetic analysis of exoenzyme S expression by Pseudomonas aeruginosa.
Genetic analysis of exoenzyme S expression by Pseudomonas aeruginosa.
复制标题
铜绿假单胞菌外切酶S表达的遗传分析。
DOI：
10.1111/j.1574-6968.1996.tb07981.x
复制
发表时间：
1996
影响因子：
2.1
通讯作者：
Frank,DW
中科院分区：
文献类型：
--
作者：
Goranson,J;Frank,DW
铜绿假单胞菌传播到血液中会增加发生致命性败血症的可能性。在实验模型中，传播的能力与胞外酶S途径的表达有关。对该途径的遗传和生化分析已经导致鉴定了编码外切酶S的两个结构基因exoSandexoT。该途径的几个位点的关键调节因子已被鉴定为具有转录激活特性的DNA结合蛋白。初步证据表明，外切酶S和耶尔森氏菌的Yop毒力决定因素之间的同源性参与其合成和分泌的蛋白质。随着exoSandexoT加入分子库，有关外切酶S的体内毒性和靶向特异性的问题可以直接得到解决。
The dissemination ofPseudomonas aeruginosato the bloodstream increases the likelihood of developing fatal sepsis. In experimental models, the ability to disseminate is linked to expression of the exoenzyme S pathway. Genetic and biochemical analysis of the pathway has led to the identification of the two structural genes encoding exoenzyme S,exoSandexoT. A key regulator of several loci of the pathway has been identified as a DNA-binding protein with transcriptional activation properties. Preliminary evidence suggests that exoenzyme S and the Yop virulence determinants of yersiniae share homology among proteins involved in their synthesis and secretion. With the addition ofexoSandexoTto the molecular arsenal, questions concerning in vivo toxicity and target specificities of exoenzyme S can be directly addressed.